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1. (WO2001002602) NUCLEOTIDES FOR CYCLE SEQUENCING OF GC-RICH TEMPLATES

Pub. No.:    WO/2001/002602    International Application No.:    PCT/EP2000/006349
Publication Date: Fri Jan 12 00:59:59 CET 2001 International Filing Date: Thu Jul 06 01:59:59 CEST 2000
IPC: C12Q 1/68
Applicants: LION BIOSCIENCE AG
MOTZ, Michael
VOSS, Hartmut
Inventors: MOTZ, Michael
VOSS, Hartmut
Title: NUCLEOTIDES FOR CYCLE SEQUENCING OF GC-RICH TEMPLATES
Abstract:
The present invention relates to a mixture comprising 7-deaza dGTP or a functional analogue thereof and dITP. The invention further relates to a method for sequencing and or amplification of a nucleic acid sequence comprising the steps of preparing a sequencing mixture comprising, among others, 7-deaza dGTP or a functional analogue thereof and dITP, further (NH4)2SO4, a nucleic acid sequence to be sequenced and/or amplified, and some enzymatic activity for incorporating dNTPs and/or ddNTPs or derivatives thereof.