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Machine translation
1. (WO2001001761) METHOD OF REGENERATING INDIVIDUAL OF PLANT BELONGING TO THE GENUS LILIUM
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2001/001761    International Application No.:    PCT/JP2000/004297
Publication Date: 11.01.2001 International Filing Date: 29.06.2000
Chapter 2 Demand Filed:    29.06.2000    
IPC:
A01H 1/02 (2006.01), A01H 4/00 (2006.01), C12N 5/14 (2006.01)
Applicants: SANKYO COMPANY, LIMITED [JP/JP]; 5-1, Nihonbashi Honcho 3-chome, Chuo-ku, Tokyo 103-8426 (JP).
HOKKAI SANKYO CO. LTD. [JP/JP]; 27-4, Kitanosato, Kita-Hiroshima-shi, Hokkaido 061-1111 (JP)
Inventors: TABAYASHI, Noriko; (JP)
Agent: NAKAMURA, Minoru; Shin-Tokyo Building, Room 646, 3-1, Marunouchi 3-chome, Chiyoda-ku, Tokyo 100-8355 (JP)
Priority Data:
11/187806 01.07.1999 JP
Title (EN) METHOD OF REGENERATING INDIVIDUAL OF PLANT BELONGING TO THE GENUS LILIUM
(FR) PROCEDE DE REGENERATION D'UNE PLANTE APPARTENANT AU GENRE LILIUM
Abstract: front page image
(EN)A method of regenerating an individual of a plant belonging to the genus Lilium which comprises the following steps: [i] inducing a callus through dedifferentiation of a tissue of the plant belonging to the genus Lilium; [ii] purifying a protoplast from the culture of the above callus; [iii] nurse-culturing the protoplast thus purified; [iv] inducing a callus from a colony of the protoplast obtained by the nurse culture; and [v] redifferentiating the callus culture. By using this method, an individual can be efficiently regenerated from a protoplast of a plant belonging to the genus Lilium of a foundation stock or a cultivated species. This regeneration method is also applicable to a fused protoplast obtained by the cell fusion method.
(FR)Cette invention concerne un procédé de régénération d'une plante du genre Lilium qui comprend les opérations suivantes : (1) obtention d'un cal par dé-différentiation d'un tissu de plante appartenant au genre Lilium); (ii) purification d'un protoplaste à partir de la culture du cal susmentionné ; (iii) culture du protoplaste ainsi purifié ; (iv)obtention d'un cal à partir d'une colonie du protoplaste obtenu par culture ; et (v) re-différentiation de la culture du cal. Ce procédé permet de régénérer efficacement un individu à partir d'un protoplaste de plante appartenant au genre Lilium de sujets de souche ou d'une espèce cultivée. Ce procédé de régénération peut également s'appliquer à un protoplaste hybridé obtenu par fusion cellulaire.
Designated States: AU, IL, NZ.
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)