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1. (WO2001001138) FLUORESCENT INTENSITY METHOD FOR ASSAYING BINDING BETWEEN PROTEINS OR PEPTIDES

Pub. No.:    WO/2001/001138    International Application No.:    PCT/IB2000/000842
Publication Date: Fri Jan 05 00:59:59 CET 2001 International Filing Date: Wed Jun 21 01:59:59 CEST 2000
IPC: G01N 33/542
Applicants: INGENEUS CORP.
Inventors: ERIKSON, Glen, H.
DAKSIS, Jasmine, I.
Title: FLUORESCENT INTENSITY METHOD FOR ASSAYING BINDING BETWEEN PROTEINS OR PEPTIDES
Abstract:
A method for assaying specific binding between a fluorophore-labeled probe and an unlabeled target is provided. The method includes detecting a quenching effect on fluorescence emitted by the fluorophore-labeled probe resulting from binding. The method is conducted without separating complexes of the target and probe from the free target and free probe prior to quenching effect detecting, and without providing a signal quenching agent to quench fluorescent light. Preferably, the probe and target are amino acid-containing compounds, such as proteins. The method can be used for a variety of applications, including screening for drug candidates having optimum binding properties, and quantifying and classifying the binding characteristics between peptide-containing compounds. The method is more sensitive than conventional assays, enabling the analysis of minute samples and low affinity binding interactions between receptors and ligands that are below the detection limits of conventional technology.