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1. (WO2001000856) HIGH EFFICIENCY TRANSFECTION BASED ON LOW ELECTRIC FIELD STRENGTH, LONG PULSE LENGTH
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2001/000856 International Application No.: PCT/US1999/014447
Publication Date: 04.01.2001 International Filing Date: 25.06.1999
Chapter 2 Demand Filed: 21.01.2000
IPC:
C12N 15/87 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Applicants:
NOLAN, Ed [US/US]; US (UsOnly)
FILSHIE, Robin [AU/CA]; CA (UsOnly)
GENETRONICS, INC. [US/US]; 11199-A Sorrento Valley Road San Diego, CA 92121-1334, US (AT, AU, BE, CA, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, JP, LU, MC, NL, PT, SE)
Inventors:
NOLAN, Ed; US
FILSHIE, Robin; CA
Agent:
HAILE, Lisa, A.; Gray Cary Ware & Freidenrich LLP Suite 1600 4365 Executive Drive San Diego, CA 92121-2189, US
Priority Data:
Title (EN) HIGH EFFICIENCY TRANSFECTION BASED ON LOW ELECTRIC FIELD STRENGTH, LONG PULSE LENGTH
(FR) TRANSFECTION TRES EFFICACE UTILISANT UNE FAIBLE INTENSITE DE CHAMP ELECTRIQUE ET UNE LONGUE DUREE D'IMPULSION
Abstract:
(EN) A method is provided for introducing nucleic acid into a cell, by contacting the cell with a nucleic acid and applying a low electrical field impulse for a long pulse length. A method is provided for introducing a polypeptide into a cell, by contacting the cell with the polypeptide and applying a low electrical field impulse for a long pulse length.
(FR) L'invention concerne un procédé permettant l'introduction d'acide nucléique dans une cellule par la mise en contact de la cellule avec un acide nucléique et l'application d'une impulsion de faible intensité de champ électrique pendant une longue durée d'impulsion. L'invention concerne aussi un procédé permettant l'introduction d'un polypeptide dans une cellule par la mise en contact de la cellule avec le polypeptide et l'application d'une impulsion de faible intensité de champ électrique pendant une longue durée d'impulsion.
Designated States: AU, CA, JP, US
European Patent Office (EPO) (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)