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1. (WO2001000642) HIGH AFFINITY, LOW MOLECULAR WEIGHT DISPLACERS FOR OLIGONUCLEOTIDE PURIFICATION

Pub. No.:    WO/2001/000642    International Application No.:    PCT/US2000/017750
Publication Date: Fri Jan 05 00:59:59 CET 2001 International Filing Date: Thu Jun 29 01:59:59 CEST 2000
IPC: B01D 15/42
C12N 15/10
Applicants: ISIS PHARMACEUTICALS, INC.
SHUKLA, Abhinav, A.
DESHMUKH, Ranjit, R.
CRAMER, Steven, M.
MOORE, James, A.
Inventors: SHUKLA, Abhinav, A.
DESHMUKH, Ranjit, R.
CRAMER, Steven, M.
MOORE, James, A.
Title: HIGH AFFINITY, LOW MOLECULAR WEIGHT DISPLACERS FOR OLIGONUCLEOTIDE PURIFICATION
Abstract:
A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.