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Machine translation
1. (WO2001000642) HIGH AFFINITY, LOW MOLECULAR WEIGHT DISPLACERS FOR OLIGONUCLEOTIDE PURIFICATION
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2001/000642    International Application No.:    PCT/US2000/017750
Publication Date: 04.01.2001 International Filing Date: 28.06.2000
Chapter 2 Demand Filed:    24.01.2001    
IPC:
B01D 15/42 (2006.01), C12N 15/10 (2006.01)
Applicants: ISIS PHARMACEUTICALS, INC. [US/US]; 2292 Faraday Avenue, Carlsbad, CA 92008 (US) (For All Designated States Except US).
SHUKLA, Abhinav, A. [IN/US]; (US) (For US Only).
DESHMUKH, Ranjit, R. [IN/US]; (US) (For US Only).
CRAMER, Steven, M. [US/US]; (US) (For US Only).
MOORE, James, A. [US/US]; (US) (For US Only)
Inventors: SHUKLA, Abhinav, A.; (US).
DESHMUKH, Ranjit, R.; (US).
CRAMER, Steven, M.; (US).
MOORE, James, A.; (US)
Agent: CALDWELL, John, W.; Woodcock Washburn Kurtz MacKiewicz & Norris LLP, 46th floor, One Liberty Place, Philadelphia, PA 19103 (US)
Priority Data:
09/343,006 29.06.1999 US
Title (EN) HIGH AFFINITY, LOW MOLECULAR WEIGHT DISPLACERS FOR OLIGONUCLEOTIDE PURIFICATION
(FR) AGENTS DE DEPLACEMENT DE BAS POIDS MOLECULAIRE, D'AFFINITE ELEVEE, POUR LA PURIFICATION D'OLIGONUCLEOTIDES
Abstract: front page image
(EN)A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.
(FR)La présente invention concerne un procédé de purification d'oligonucléotides par chromatographie de déplacement sur un support échangeur d'anions, au moyen d'agents de déplacement de bas poids moléculaire (inférieur à 100000 Da), d'affinité élevée. Elle concerne aussi plusieurs exemples d'agents de déplacements anioniques de bas poids moléculaire, d'affinité élevée, comprenant des composés aromatiques polycycliques comportant des résidus acide sulfonique. On démontre l'efficacité de la technique pour une séparation haute résolution d'oligonucléotides à partir d'un mélange industriel.
Designated States: AE, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CR, CU, CZ, DE, DK, DM, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)