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Machine translation
1. (WO2000063403) RECOMBINANT PROTEIN PRODUCTION IN A HUMAN CELL USING SEQUENCES ENCODING ADENOVIRUS E1 PROTEIN
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2000/063403    International Application No.:    PCT/NL2000/000247
Publication Date: 26.10.2000 International Filing Date: 17.04.2000
Chapter 2 Demand Filed:    13.11.2000    
IPC:
C12N 15/85 (2006.01)
Applicants: CRUCELL HOLLAND B.V. [NL/NL]; Archimedesweg 4, NL-2333 CN Leiden (NL) (For All Designated States Except US).
HATEBOER, Guus [NL/NL]; (NL) (For US Only).
VERHULST, Karina, Cornelia [NL/NL]; (NL) (For US Only).
SCHOUTEN, Govert, Johan [NL/NL]; (NL) (For US Only).
UYTDEHAAG, Alphonsus, Gerardus, Cornelis, Maria [NL/NL]; (NL) (For US Only).
BOUT, Abraham [NL/NL]; (NL) (For US Only)
Inventors: HATEBOER, Guus; (NL).
VERHULST, Karina, Cornelia; (NL).
SCHOUTEN, Govert, Johan; (NL).
UYTDEHAAG, Alphonsus, Gerardus, Cornelis, Maria; (NL).
BOUT, Abraham; (NL)
Agent: PRINS, A., W.; Vereenigde, Nieuwe Parklaan 97, NL-2587 BN The Hague (NL)
Priority Data:
99201176.7 15.04.1999 EP
99204434.7 21.12.1999 EP
Title (EN) RECOMBINANT PROTEIN PRODUCTION IN A HUMAN CELL USING SEQUENCES ENCODING ADENOVIRUS E1 PROTEIN
(FR) PRODUCTION DE PROTEINE DE RECOMBINAISON DANS UNE CELLULE HUMAINE
Abstract: front page image
(EN)The present invention provides methods and compositions for the production of recombinant proteins in a human cell line, using sequences encoding at least one E1 protein of an adenovirus where the cells does not encode a structural adenoviral protein from its genome. The methods and compositions are particularly useful for generating stable expression of human recombinant proteins of interest that are modified post-translationally, e.g. by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems like Chinese Hamster Ovary (CHO) cells.
(FR)Ces méthodes et les compositions correspondantes, permettant de produire des protéines de recombinaison dans une lignée cellulaire, se révèlent des plus utiles pour exprimer de façon stable des protéines humaines de recombinaison modifiées après translation, par exemple par glycosylation. Ces protéines ont des caractéristiques supérieures comparé à leurs homologues produites dans des systèmes non humain, notamment dans les cellules ovariennes de hamster chinois.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CR, CU, CZ, DE, DK, DM, DZ, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)