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1. (WO2000058484) TRANSFORMATION OF MONOCOTYLEDONEOUS PLANTS USING AGROBACTERIUM
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2000/058484    International Application No.:    PCT/CA2000/000306
Publication Date: 05.10.2000 International Filing Date: 24.03.2000
Chapter 2 Demand Filed:    24.10.2000    
IPC:
C12N 9/02 (2006.01), C12N 15/82 (2006.01)
Applicants: UNIVERSITY OF GUELPH [CA/CA]; Reynolds Building, Suite 213, Office of Research, Guelph, Ontario N1G 2W1 (CA) (For All Designated States Except US).
ROJAS, Brenda [CA/CA]; (CA) (For US Only).
BOWLEY, Stephen, R. [CA/CA]; (CA) (For US Only).
DEVEREAUX, Alissa [CA/CA]; (CA) (For US Only)
Inventors: ROJAS, Brenda; (CA).
BOWLEY, Stephen, R.; (CA).
DEVEREAUX, Alissa; (CA)
Agent: SECHLEY, Konrad, A.; Gowling Lafleur Henderson LLP., Suite 2600, 160 Elgin Street, Ottawa, Ontario K1P 1C3 (CA)
Priority Data:
2,267,014 26.03.1999 CA
Title (EN) TRANSFORMATION OF MONOCOTYLEDONEOUS PLANTS USING AGROBACTERIUM
(FR) TRANSFORMATION DE PLANTES MONOCOTYLEDONES A L'AIDE D'AGROBACTERIUM
Abstract: front page image
(EN)The present invention is directed to a method for the transformation of a monocot plant comprising exposing explant tissue of said monocot to an Agrobacterium strain under vacuum in the presence of a phenolic compound, said Agrobacterium strain comprising a heterologous gene of interest within a vector. The Agrobacterium is removed from the explant tissue, and an antibiotic against the Agrobacterium is added, and the transformed tissue is plated onto growth medium, grown, and then plated onto selection media. The method may be used with vectors comprising either a vir+ vector, or a super virulent vector.
(FR)L'invention porte sur un procédé de transformation de plantes monocotylédones consistant à en exposer les tissus à une souche d'$i(Agrobacterium),$i( )sous vide et en présence d'un composé phénolique, ladite souche comprenant un gène hétérologue d'intérêt compris dans un vecteur. On retire ensuite l'$i(Agrobacterium)) du tissu, puis on ajoute un antibiotique anti-$i(Agrobacterium), puis on place le tissu transformé sur un milieu de culture, on le fait croître, puis on le place sur un milieu de sélection. Le procédé peut servir avec des vecteurs soit du type vir+ soit du type supervirulent.
Designated States: AE, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CR, CU, CZ, DE, DK, DM, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)