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1. (WO2000057982) FLUIDISED BED PURIFICATION OF BIO-MACROMOLECULES SUCH AS PLASMID DNA, CHROMOSOMAL DNA, RNA, VIRAL DNA, BACTERIA AND VIRUSES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2000/057982    International Application No.:    PCT/DK2000/000142
Publication Date: 05.10.2000 International Filing Date: 24.03.2000
Chapter 2 Demand Filed:    13.10.2000    
IPC:
B01D 15/02 (2006.01), B01D 15/08 (2006.01), B01J 20/28 (2006.01), B01J 20/32 (2006.01), C12N 15/10 (2006.01)
Applicants: UPFRONT CHROMATOGRAPHY A/S [DK/DK]; Lersø Parkallé 42, DK-2100 Copenhagen Ø (DK) (For All Designated States Except US).
OLANDER, Morten, Aae [DK/DK]; (DK) (For US Only).
LIHME, Allan, Otto, Fog [DK/DK]; (DK) (For US Only).
HOBLEY, Timothy, John [AU/DK]; (DK) (For US Only).
SIMON, Marcos [ES/DK]; (DK) (For US Only).
THEODOSSIOU, Irini [GR/DK]; (DK) (For US Only).
THOMAS, Owen, Robert, Tyrynis [GB/DK]; (DK) (For US Only)
Inventors: OLANDER, Morten, Aae; (DK).
LIHME, Allan, Otto, Fog; (DK).
HOBLEY, Timothy, John; (DK).
SIMON, Marcos; (DK).
THEODOSSIOU, Irini; (DK).
THOMAS, Owen, Robert, Tyrynis; (DK)
Agent: PLOUGMANN & VINGTOFT A/S; Sankt Annæ Plads 11, P.O. Box 3007, DK-1021 Copenhagen K (DK)
Priority Data:
PA 1999 00415 26.03.1999 DK
Title (EN) FLUIDISED BED PURIFICATION OF BIO-MACROMOLECULES SUCH AS PLASMID DNA, CHROMOSOMAL DNA, RNA, VIRAL DNA, BACTERIA AND VIRUSES
(FR) PURIFICATION EN LIT FLUIDISE DE BIO-MACROMOLECULES TELLES QU'UN ADN PLASMIDIQUE, UN ADN CHROMOSOMIQUE, UN ARN, UN ADN VIRAL, DES BACTERIES ET DES VIRUS
Abstract: front page image
(EN)The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 $g(m)m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidised bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
(FR)L'invention concerne des matières particulaires présentant une densité d'au moins 2,5 g/ml, où les particules présentent un diamètre moyen de 5-75 $g(m)m, et sont essentiellement constituées d'une matrice de base polymère, notamment un polysaccharide tel que l'agarose, et une matière noyau non poreuse, notamment de l'acier et du titane, ladite matière noyau présentant une densité d'au moins 3,0 g/ml, ladite matrice de base polymère comprenant des groupes latéraux qui sont chargés positivement à un pH de 4,0 ou qui sont des ligands d'affinité pour une bio-molécule. Des groupes latéraux possibles sont la polyéthylènéimine (PEI), le diéthylaminoéthyle (DEAE) et l'aminoéthyle quaternaire (QAE). Ces matières sont utiles pour des procédés chromatographiques en lit expansé ou en lit fluidisé, notamment pour la purification de bio-macromolécules telles qu'un ADN plasmidique, un ADN chromosomique, un ARN, un ADN viral, des bactéries et des virus.
Designated States: AE, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CR, CU, CZ, DE, DK, DM, DZ, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)