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1. (WO2000050642) SEQUENCING BY INCORPORATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2000/050642 International Application No.: PCT/US2000/004486
Publication Date: 31.08.2000 International Filing Date: 22.02.2000
Chapter 2 Demand Filed: 01.09.2000
IPC:
B01F 13/00 (2006.01) ,B01L 3/00 (2006.01) ,C07H 21/02 (2006.01) ,C07H 21/04 (2006.01) ,G01N 27/447 (2006.01) ,G01N 35/00 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
F
MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
13
Other mixers; Mixing plant, including combinations of dissimilar mixers
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
L
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3
Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02
with ribosyl as saccharide radical
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04
with deoxyribosyl as saccharide radical
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26
by investigating electrochemical variables; by using electrolysis or electrophoresis
416
Systems
447
using electrophoresis
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35
Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
Applicants:
CALIPER TECHNOLOGIES CORP. [US/US]; 605 Fairchild Drive Mountain View, CA 94043, US (AllExceptUS)
PARCE, J., Wallace [US/US]; US (UsOnly)
NIKIFOROV, Theo, T. [US/US]; US (UsOnly)
BURD MEHTA, Tammy [US/US]; US (UsOnly)
KOPF-SILL, Anne [US/US]; US (UsOnly)
CHOW, Andrea, W. [US/US]; US (UsOnly)
KNAPP, Michael, R. [US/US]; US (UsOnly)
Inventors:
PARCE, J., Wallace; US
NIKIFOROV, Theo, T.; US
BURD MEHTA, Tammy; US
KOPF-SILL, Anne; US
CHOW, Andrea, W.; US
KNAPP, Michael, R.; US
Agent:
QUINE, Jonathan, Alan; The Law Offices of Jonathan Alan Quine P.O. Box 458 Alameda, CA 94501 , US
Priority Data:
60/121,22323.02.1999US
60/127,82505.04.1999US
60/128,64309.04.1999US
null22.02.2000US (Considered void 10.10.2002)
null22.02.2000US (Considered void 10.10.2002)
null22.02.2000US (Considered void 10.10.2002)
Title (EN) SEQUENCING BY INCORPORATION
(FR) SEQUENÇAGE PAR INCORPORATION
Abstract:
(EN) Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3'-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Mircrofluidic devices, including particle arrays, are used in the sequencing methods.
(FR) L'invention concerne des nucléotides et des analogues de nucléotides utilisés dans différents séquençages par incorporation/séquençage au moyen de techniques de synthèse. Des analogues de nucléotide comprenant des groupes bloquants 3' sont utilisés pour produire une terminaison de chaîne réversible pour séquençage par synthèse. Des groupes bloquants typiques comprennent des groupes phosphate et des groupes carbamate. Des nucléotides fluorescents sont utilisés pour effectuer un séquençage par synthèse avec détection par incorporation du nucléotide marqué par fluorescence, éventuellement suive d'un photoblanchiment d'une insertion de colorants utilisés pour détecter l'adjonction d'un nucléotide non marqué dans un séquençage par synthèse avec détection par insertion. On utilise des dispositifs microfluidiques, comprenant des réseaux de particules, dans ces techniques de séquençage.
Designated States: AE, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CR, CU, CZ, DE, DK, DM, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1163369AU2000034989