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1. WO2000050642 - SEQUENCING BY INCORPORATION

Publication Number WO/2000/050642
Publication Date 31.08.2000
International Application No. PCT/US2000/004486
International Filing Date 22.02.2000
Chapter 2 Demand Filed 01.09.2000
IPC
B01F 13/00 2006.1
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
13Other mixers; Mixing plant, including combinations of dissimilar mixers
B01L 3/00 2006.1
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C07H 21/02 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02with ribosyl as saccharide radical
C07H 21/04 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04with deoxyribosyl as saccharide radical
G01N 27/447 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
G01N 35/00 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
CPC
B01F 33/30
B01J 2219/00317
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00277Apparatus
00279Features relating to reactor vessels
00306Reactor vessels in a multiple arrangement
00313the reactor vessels being formed by arrays of wells in blocks
00315Microtiter plates
00317Microwell devices, i.e. having large numbers of wells
B01J 2219/00459
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00277Apparatus
00457Dispensing or evacuation of the solid phase support
00459Beads
B01J 2219/00468
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00277Apparatus
00457Dispensing or evacuation of the solid phase support
00459Beads
00468by manipulation of individual beads
B01L 2200/0668
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
06Fluid handling related problems
0647Handling flowable solids, e.g. microscopic beads, cells, particles
0668Trapping microscopic beads
B01L 2400/0409
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0409centrifugal forces
Applicants
  • CALIPER TECHNOLOGIES CORP. [US]/[US] (AllExceptUS)
  • PARCE, J., Wallace [US]/[US] (UsOnly)
  • NIKIFOROV, Theo, T. [US]/[US] (UsOnly)
  • BURD MEHTA, Tammy [US]/[US] (UsOnly)
  • KOPF-SILL, Anne [US]/[US] (UsOnly)
  • CHOW, Andrea, W. [US]/[US] (UsOnly)
  • KNAPP, Michael, R. [US]/[US] (UsOnly)
Inventors
  • PARCE, J., Wallace
  • NIKIFOROV, Theo, T.
  • BURD MEHTA, Tammy
  • KOPF-SILL, Anne
  • CHOW, Andrea, W.
  • KNAPP, Michael, R.
Agents
  • QUINE, Jonathan, Alan
Priority Data
60/121,22323.02.1999US
60/127,82505.04.1999US
60/128,64309.04.1999US
null22.02.2000US (Considered void 10.10.2002)
null22.02.2000US (Considered void 10.10.2002)
null22.02.2000US (Considered void 10.10.2002)
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SEQUENCING BY INCORPORATION
(FR) SEQUENÇAGE PAR INCORPORATION
Abstract
(EN) Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3'-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Mircrofluidic devices, including particle arrays, are used in the sequencing methods.
(FR) L'invention concerne des nucléotides et des analogues de nucléotides utilisés dans différents séquençages par incorporation/séquençage au moyen de techniques de synthèse. Des analogues de nucléotide comprenant des groupes bloquants 3' sont utilisés pour produire une terminaison de chaîne réversible pour séquençage par synthèse. Des groupes bloquants typiques comprennent des groupes phosphate et des groupes carbamate. Des nucléotides fluorescents sont utilisés pour effectuer un séquençage par synthèse avec détection par incorporation du nucléotide marqué par fluorescence, éventuellement suive d'un photoblanchiment d'une insertion de colorants utilisés pour détecter l'adjonction d'un nucléotide non marqué dans un séquençage par synthèse avec détection par insertion. On utilise des dispositifs microfluidiques, comprenant des réseaux de particules, dans ces techniques de séquençage.
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