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1. (WO2000049150) CHIMERIC GENE CONSTRUCTS FOR GENERATION OF FLUORESCENT TRANSGENIC ORNAMENTAL FISH

Pub. No.:    WO/2000/049150    International Application No.:    PCT/SG1999/000079
Publication Date: Fri Aug 25 01:59:59 CEST 2000 International Filing Date: Sat Jul 17 01:59:59 CEST 1999
IPC: C07K 14/435
C12N 15/85
G01N 33/18
G01N 33/74
Applicants: NATIONAL UNIVERSITY OF SINGAPORE
XU, Yianfei
HE, Jiangyan
YAN, Tie
GONG, Zhiyuan
LAM, Toong, Jin
JU, Bensheng
Inventors: GONG, Zhiyuan
LAM, Toong, Jin
JU, Bensheng
Title: CHIMERIC GENE CONSTRUCTS FOR GENERATION OF FLUORESCENT TRANSGENIC ORNAMENTAL FISH
Abstract:
Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5' end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.