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1. WO2000049150 - CHIMERIC GENE CONSTRUCTS FOR GENERATION OF FLUORESCENT TRANSGENIC ORNAMENTAL FISH

Publication Number WO/2000/049150
Publication Date 24.08.2000
International Application No. PCT/SG1999/000079
International Filing Date 16.07.1999
Chapter 2 Demand Filed 18.01.2000
IPC
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
18
Water
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
74
involving hormones
C07K 14/435 (2006.01)
C12N 15/85 (2006.01)
G01N 33/18 (2006.01)
G01N 33/74 (2006.01)
CPC
A01K 2217/05
A01K 2217/20
A01K 2227/40
A01K 2267/02
A01K 2267/0393
A01K 67/0275
Applicants
  • NATIONAL UNIVERSITY OF SINGAPORE [SG/SG]; 10 Kent Ridge Crescent Singapore 119260, SG (AllExceptUS)
  • XU, Yianfei [CN/SG]; SG (AllExceptUS)
  • HE, Jiangyan [CN/SG]; SG (AllExceptUS)
  • YAN, Tie [CN/SG]; SG (AllExceptUS)
  • GONG, Zhiyuan [CA/SG]; SG (UsOnly)
  • LAM, Toong, Jin [SG/SG]; SG (UsOnly)
  • JU, Bensheng [CN/SG]; SG (UsOnly)
Inventors
  • GONG, Zhiyuan; SG
  • LAM, Toong, Jin; SG
  • JU, Bensheng; SG
Agents
  • SURESAN, Sachithananthan; C/O Tan Rajah & Cheah 9 Battery Road, #15-00 Straits Trading Building Singapore 049910, SG
Priority Data
9900811-218.02.1999SG
null14.07.1999SG
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CHIMERIC GENE CONSTRUCTS FOR GENERATION OF FLUORESCENT TRANSGENIC ORNAMENTAL FISH
(FR) CONSTRUCTIONS GENIQUES PERMETTANT DE PRODUIRE UN POISSON D'AGREMENT TRANSGENIQUE FLUORESCENT
Abstract
(EN)
Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5' end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.
(FR)
L'invention concerne quatre promoteurs géniques du poisson zèbre, spécifiques de la peau, des muscles, et des muscles du squelette, exprimés respectivement de manière ubiquiste, isolés et formant une ligature avec l'extrémité 5' du gène de la protéine fluorescente verte améliorée (EGFP). Lorsque les constructions géniques chimères résultantes sont introduites dans le poisson zèbre, le poisson zèbre transgénique émet une fluorescence verte en réponse à une lumière bleue ou ultraviolette, en fonction de la spécificité des promoteurs utilisés. Puis, on développe de nouvelles variétés de poissons d'agrément présentant différents motifs fluorescents, par exemple la fluorescence de la peau, des muscles, et la fluorescence spécifique d'un muscle du squelette et/ou ubiquiste.
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