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1. WO2000043819 - CONFOCAL MICROSCOPY APPARATUS AND METHOD

Publication Number WO/2000/043819
Publication Date 27.07.2000
International Application No. PCT/GB2000/000142
International Filing Date 17.01.2000
Chapter 2 Demand Filed 03.08.2000
IPC
G02B 21/00 2006.01
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
CPC
G02B 21/0024
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
G02B 21/0052
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
0052Optical details of the image generation
G02B 21/0056
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
0052Optical details of the image generation
0056based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
G02B 21/0068
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
0052Optical details of the image generation
0068arrangements using polarisation
G02B 21/0076
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
0052Optical details of the image generation
0076arrangements using fluorescence or luminescence
G02B 21/0084
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
008Details of detection or image processing, including general computer control
0084time-scale detection, e.g. strobed, ultra-fast, heterodyne detection
Applicants
  • ISIS INNOVATION LIMITED [GB/GB]; Ewert House Ewert Place Summertown, Oxford Oxfordshire OX2 7BZ Uniteed Kingdom, GB (AllExceptUS)
  • WILSON, Tony [GB/GB]; GB (UsOnly)
  • NEIL, Mark, Andrew, Aquilla [GB/GB]; GB (UsOnly)
  • JUSKAITIS, Rimvydas [LT/GB]; GB (UsOnly)
Inventors
  • WILSON, Tony; GB
  • NEIL, Mark, Andrew, Aquilla; GB
  • JUSKAITIS, Rimvydas; GB
Agents
  • PERKINS, Sarah ; Stevens, Hewlett & Perkins Halton House 20/23 Holborn London EC1N 2JD, GB
Priority Data
9901365.822.01.1999GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CONFOCAL MICROSCOPY APPARATUS AND METHOD
(FR) APPAREIL DE MICROSCOPIE CONFOCALE ET PROCEDE ASSOCIE
Abstract
(EN)
The confocal microscope has two matched light sources: a first light source (1) and a second light source (8). The light sources (1,8) are arranged to illuminate opposite sides of a modulating mask (6). The light either reflecting from or passing through the modulating mask (6) is then used to illuminate an object O supported on a mount (5). The microscope is arranged so that the object O is mounted on the opposite side of the mask (6) to a camera (7) such that light reflected from the object O passes through the modulating mask (6) before being captured by the camera (7). Subtraction of the image produced using the second light source (8) from the image produced using the first light source (1) generates a confocal image that contains substantially less noise than is possible with available confocal microscopy apparatus.
(FR)
Le microscope confocal selon la présente invention comprend deux sources de lumière assorties telles qu'une première source (1) de lumière et une deuxième source (8) de lumière. Les sources (1, 8) de lumière sont disposées de manière à éclairer les côtés opposés d'un masque (6) de modulation. La lumière qui est réfléchie par le masque (6) de modulation ou qui traverse ce dernier est ensuite utilisée pour éclairer un objet O placé sur un support (5). Le microscope est disposé de sorte que l'objet O soit placé du côté du masque (6) qui est opposé à une caméra (7) afin que la lumière réfléchie par l'objet O traverse le masque (6) de modulation avant d'être captée par la caméra (7). Pour obtenir une image confocale contenant sensiblement moins de bruit que ce qu'il est possible d'avoir avec un appareil de microscopie confocale existant à ce jour, on soustrait l'image produite à l'aide de la deuxième source (8) de lumière, de l'image produite à l'aide de la première source (1) de lumière.
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