Processing

Please wait...

Settings

Settings

1. WO2000043785 - IMPROVEMENTS IN OR RELATING TO DISPLACEMENT ASSAYS

Publication Number WO/2000/043785
Publication Date 27.07.2000
International Application No. PCT/EP1999/010456
International Filing Date 23.12.1999
Chapter 2 Demand Filed 05.06.2000
IPC
G01N 33/543 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
CPC
G01N 2333/575
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
575Hormones
G01N 2333/59
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
575Hormones
59Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
G01N 33/54306
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54306Solid-phase reaction mechanisms
Applicants
  • UNILEVER PLC [GB/GB]; Unilever House Blackfriars London EC4P 4BQ, GB (AE, AU, BB, CA, CY, GB, GD, GH, GM, IE, IL, KE, LC, LK, LS, MN, MW, NZ, SD, SG, SL, SZ, TT, TZ, UG, ZA, ZW)
  • UNILEVER NV [NL/NL]; Weena 455 NL-3013 AL Rotterdam, NL (AL, AM, AT, AZ, BA, BE, BF, BG, BJ, BR, BY, CF, CG, CH, CI, CM, CN, CR, CU, CZ, DE, DK, DM, EE, ES, FI, FR, GA, GE, GN, GR, GW, HR, HU, ID, IS, IT, JP, KG, KP, KR, KZ, LR, LT, LU, LV, MA, MC, MD, MG, MK, ML, MR, MX, NE, NL, NO, PL, PT, RO, RU, SE, SI, SK, SN, TD, TG, TJ, TM, TR, UA, UZ, VN, YU)
  • HINDUSTAN LEVER LIMITED [IN/IN]; Hindustan Lever House 165/166 Backbay Reclamation Maharashtra 400 020 Mumbai, IN (IN)
Inventors
  • HOWELL, Steven; GB
  • NEBE VON CARON, Gerhard; GB
Agents
  • EVANS, Jacqueline, Gail, Victoria ; Unilever PLC, Patent Department Colworth House Sharnbrook Bedford, Bedfordshire MK44 1LQ, GB
Priority Data
99300518.025.01.1999EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) IMPROVEMENTS IN OR RELATING TO DISPLACEMENT ASSAYS
(FR) AMELIORATIONS APPORTEES A DES DOSAGES PAR DEPLACEMENT
Abstract
(EN)
A method and assay device for performing displacement assays having increased sensitivity. In particular, the assay device includes a first solid support having reversibly immobilised thereon a binding partner having specific binding activity for an analyte of interest, the binding partner capable of being displaced from the first solid support in the presence of the analyte of interest and forming a binding partner/analyte complex; a second solid support bearing a capture moiety, the capture moiety having a specific binding affinity for the binding partner which is greater than that of the analyte of interest for the binding partner, such that the binding partner may be captured by the capture moiety and the analyte of interest displaced from the binding partner/analyte complex; means for conducting the binding partner/analyte complex from the first solid support to the second solid support; and a means for conducting the displaced analyte from the second solid support to the first solid support.
(FR)
Cette invention se rapporte à un procédé et à un dispositif de dosage permettant de réaliser des dosages par déplacement avec une sensibilité accrue. Ce dispositif de dosage comprend en particulier un premier support solide sur lequel est immobilisé de façon réversible un partenaire de liaison possédant une activité de liaison spécifique pour un analyte d'intérêt, ce partenaire de liaison pouvant être déplacé depuis le premier support solide en présence de l'analyte d'intérêt et formant un complexe partenaire de liaison/analyte; un second support solide portant une fraction de capture, laquelle possède une affinité de liaison spécifique pour le partenaire de liaison qui est supérieure à celle de l'analyte d'intérêt pour le partenaire de liaison, de telle sorte que le partenaire de liaison puisse être capturé par la fraction de capture et l'analyte d'intérêt déplacé depuis le complexe partenaire de liaison/analyte; un organe permettant de guider le complexe partenaire de liaison/analyte depuis le premier support solide jusqu'au second support solide; et un organe permettant de guider l'analyte ainsi déplacé depuis le second support solide jusqu'au premier support solide.
Also published as
Latest bibliographic data on file with the International Bureau