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1. WO2000043756 - BLOOD METHEMOGLOBIN ANALYSIS

Publication Number WO/2000/043756
Publication Date 27.07.2000
International Application No. PCT/US2000/000546
International Filing Date 10.01.2000
Chapter 2 Demand Filed 15.08.2000
IPC
G01N 33/72 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
72involving blood pigments, e.g. hemoglobin, bilirubin
CPC
G01N 33/721
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
72involving blood pigments, e.g. haemoglobin, bilirubin ; or other porphyrins; involving occult blood
721Haemoglobin
Y10T 436/10
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
Y10T 436/107497
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
107497Preparation composition [e.g., lysing or precipitation, etc.]
Y10T 436/108331
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
108331Preservative, buffer, anticoagulant or diluent
Y10T 436/2525
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
25including sample preparation
2525Stabilizing or preserving
Applicants
  • THE FREDRIC RIEDERS FAMILY RENAISSANCE FOUNDATION [US/US]; 2850 Rushland Road Rushland, PA 18956, US
Inventors
  • RIEDERS, Fredric; US
  • MACHERONE, Anthony; US
Agents
  • MONACO, Daniel, A.; Drinker Biddle & Reath LLP One Logan Square 18th and Cherry Streets Philadelphia, PA 19103-6996, US
Priority Data
09/235,47222.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BLOOD METHEMOGLOBIN ANALYSIS
(FR) ANALYSE DE METHEMOGLOBINE CONTENUE DANS LE SANG
Abstract
(EN)
Blood samples are stabilized for methemoglobin determination with a buffer composition comprising (a) carbon monoxide-containing water; (b) sodium tetraborate or potassium tetraborate; and (c) KCN or NaCN. The buffer composition preferably further comprises an erythrocytolysis agent. The buffer fixes the valence state of heme iron at a concentration representative of the blood at the time of collection, and maintains that fixed state for an extended period of time, preventing further methemoglobin formation. The buffer also prevents reduction of then existing target analyte, i.e., methemoglobin (ferric hemoglobin, Fe3+ hemoglobin) to normal reduced hemoglobin (ferrous hemoglobin, Fe2+ hemoglobin).
(FR)
La présente invention concerne l'analyse de méthémoglobine contenue dans des prélèvements de sang stabilisés par une composition tampon qui comprend (a) de l'eau contenant du monoxyde de carbone, (b) du tétraborate de sodium ou du tétraborate de potassium, et (c) KCN ou NaCN. La composition tampon comprend également, de préférence, un agent d'érythrocytolyse. Le tampon fixe l'état de la valence de fer hémique à une concentration qui représente l'état du sang au moment du prélèvement, et maintient cet état fixe pendant une période prolongée, empêchant une plus grande formation de méthémoglobine. Le tampon empêche également que l'analyte ciblé existant soit réduit, c'est-à-dire que la méthémoglobine (hémoglobine ferrique, hémoglobine Fe3+) soit réduite à l'état d'hémoglobine normale (hémoglobine ferreuse, hémoglobine Fe2+).
Also published as
Latest bibliographic data on file with the International Bureau