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1. WO2000043547 - DNA MOBILITY MODIFIER

Publication Number WO/2000/043547
Publication Date 27.07.2000
International Application No. PCT/US2000/001433
International Filing Date 20.01.2000
Chapter 2 Demand Filed 07.08.2000
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
B82Y 30/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
30Nanotechnology for materials or surface science, e.g. nanocomposites
C07H 19/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
19Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides ; Anhydro-derivatives thereof
C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C07H 21/02
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02with ribosyl as saccharide radical
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
Applicants
  • NORTHWESTERN UNIVERSITY [US/US]; 1880 Oak Street, Suite 100 Evanston, IL 60201, US
Inventors
  • BARRON, Annelise, E.; US
Agents
  • NAPOLI, James, J.; Marshall, O'Toole, Gerstein, Murray & Borun 6300 Sears Tower 233 South Wacker Drive Chicago, IL 60606, US
  • CONNELL, Gary, J.; Sheridan Ross P.C. Suite 1200 1560 Broadway Denver, CO 80202-5141, US
Priority Data
60/116,78820.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DNA MOBILITY MODIFIER
(FR) MODIFICATEUR DE LA MOBILITE DE L'ADN
Abstract
(EN)
Polyamides comprising at least one hydrophilic C1-C10 hydrocarbyl substituent on an amide nitrogen atom, and methods for producing and using the same is provided. In particular, polyamides of formula (I) or (II) and methods for using the same for altering the ratio of charge/translational frictional drag of binding polymers to allow electrophoretic separation of polynucleotides or analogs thereof in a non-sieving liquid medium is provided, where a, q, L1, P1, Q1, R, R1, R10 and R11 are those described herein.
(FR)
La présente invention concerne des polyamides comprenant au moins un substituent hydrocarbyle C1-C10 hydrophile sur un atome d'azote amine, ainsi que leurs procédés de production et d'utilisation. Plus particulièrement, cette invention concerne les polyamides de formule (I) ou (II) et leurs procédés de modification du rapport de résistance de frottement de translation/charge des polymères de liaison, permettant d'effectuer la séparation électrophorétique de polynucléotides ou de leurs analogues dans un milieu liquide sans tamisage, où a, q, L1, P1, Q1, R, R1, R10 et R11 sont tels que décrits.
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