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1. WO2000043541 - SELECTION PROCEDURE USING PRODRUG/ENZYME SYSTEM

Publication Number WO/2000/043541
Publication Date 27.07.2000
International Application No. PCT/GB2000/000157
International Filing Date 21.01.2000
Chapter 2 Demand Filed 16.08.2000
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/1082
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1082Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
C12Q 1/6811
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6811Selection methods for production or design of target specific oligonucleotides or binding molecules
Applicants
  • COBRA THERAPEUTICS LIMITED [GB/GB]; University of Keele The Science Park Keele Staffordshire ST5 5SP, GB (AllExceptUS)
  • SEARLE, Peter, F. [GB/GB]; GB (UsOnly)
Inventors
  • SEARLE, Peter, F.; GB
Agents
  • HARRISON GODDARD FOOTE; Tower House Merrion Way Leeds LS2 8PA, GB
Priority Data
60/116,92422.01.1999US
9901471.422.01.1999GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SELECTION PROCEDURE USING PRODRUG/ENZYME SYSTEM
(FR) PROCEDE DE SELECTION PAR SYSTEME DE PROMEDICAMENTS/ENZYMES
Abstract
(EN)
The invention relates to a process for the selection from a gene library of a gene encoding an enzyme that is capable of catalysing the conversion of a prodrug to its active drug form. The method comprises contacting a library of lysogenic bacteria with a prodrug that causes activation of bacterial RecA when converted to its active drug form. Activation of RecA causes lysis of the bacteria, so allowing separation of bacteriophage particles released into the medium, and their subsequent genotypic analysis to isolate nucleic acid molecules in the library that encode a desired prodrug-activating enzyme.
(FR)
Cette invention concerne un procédé permettant de sélectionner, dans une banque de gènes, un gène codant pour une enzyme capable de catalyser la transformation d'un promédicament en son principe actif. Ce procédé consiste à mettre en contact une banque de bactéries lysogéniques avec un promédicament qui active la protéine RecA bactérienne lorsqu'elle se transforme en son principe actif. L'activation de la protéine RecA provoque une lyse de la bactérie, ce qui permet de séparer les particules bactériophages libérées dans le vecteur, et par ailleurs de procéder à leur analyse génotypique ultérieure afin d'isoler dans la banque des molécules d'acides nucléiques codant pour l'enzyme qui active le promédicament.
Also published as
US09889761
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