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1. WO2000043536 - GLUCOSE SENSING USING METAL-LIGAND COMPLEXES

Publication Number WO/2000/043536
Publication Date 27.07.2000
International Application No. PCT/US2000/001716
International Filing Date 21.01.2000
Chapter 2 Demand Filed 15.08.2000
IPC
G01N 33/66 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
66involving blood sugars, e.g. galactose
CPC
G01N 33/66
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
66involving blood sugars, e.g. galactose
Applicants
  • UNIVERSITY OF MARYLAND, BALTIMORE [US/US]; Office of Research & Development 520 West Lombard Street Baltimore, MD 21201-1627, US (AllExceptUS)
  • LAKOWICZ, Joseph, R. [US/US]; US (UsOnly)
  • MURTAZA, Zakir [IN/US]; US (UsOnly)
Inventors
  • LAKOWICZ, Joseph, R.; US
  • MURTAZA, Zakir; US
Agents
  • BRUMLIK, Charles, J.; Mathews, Collins, Shepherd & Gould, P.A. Suite 306 100 Thanet Circle Princeton, NJ 08540-3674, US
Priority Data
60/116,96822.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) GLUCOSE SENSING USING METAL-LIGAND COMPLEXES
(FR) DETECTION DE GLUCOSE A L'AIDE DE COMPLEXES METAUX-LIGANDS
Abstract
(EN)
Optical sensing of saccharides using a luminescent ruthenium, rhenium or osmium metal-ligand complex and a substituted phenylboronic acid is described. The preferred embodiment is a glucose sensor based on [Ru(bpy)2(dphen)](PF6)2 (where bpy is 2,2'-bipyridine and dphen is 5,6-dihydroxy-1,10-phenanthroline) and 2-bromophenylboronic acid BBA. In the presence of glucose cyclic-ester forms equilibrium between glucose and BBA. With increasing concentrations of glucose the free ruthenium complex is formed. The emission intensity of the free [Ru2+(bpy)2(dphen)2-]0 complex can be used to determine the concentration of glucose. The absorption maxima at 440 nm and long lifetime near 370 ns allows low cost glucose monitoring by using simple instrumentation using LED and phase fluorometry. The figure shows a main reaction mechanism for a first embodiment of this invention, named as Scheme 1.
(FR)
La présente invention concerne la détection optique de saccharides à l'aide d'un complexe métal ruthénium, rhénium ou osmium-ligand luminescent et d'un acide phénylborique substitué. Dans un mode de réalisation préféré, l'invention concerne un détecteur de glucose basé sur un [Ru(bpy)2(dphen)](PF6)2 (où bpy est une 2,2'-bipyridine et dphen est une 5,6 dihydroxy-1,10-phénanthroline) et sur un acide 2-bromophénylborique (BBA). En présence de glucose, l'ester cyclique forme un équilibre entre le glucose et le BBA. Des concentrations plus élevées de glucose entraînent la formation d'un complexe ruthénium libre. On peut utiliser l'intensité d'émission du complexe [Ru2+(bpy)2(dphen)2-]0 libre pour mesurer la concentration de glucose. Des maximums d'absorption à 440 nm et une longue durée de vie de près de 370 ns permettent de contrôler le glucose à moindre coût à l'aide d'un instrument simple à DEL et fluorimétrie de phase. La figure illustre le mécanisme de réaction principal d'un premier mode de réalisation de l'invention, baptisé schéma 1.
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