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1. WO2000043526 - MAMMALIAN SERINE RACEMASE

Publication Number WO/2000/043526
Publication Date 27.07.2000
International Application No. PCT/US2000/000938
International Filing Date 18.01.2000
Chapter 2 Demand Filed 07.08.2000
IPC
C12N 9/90 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
90Isomerases (5.)
CPC
C12N 9/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
90Isomerases (5.)
Applicants
  • THE JOHNS HOPKINS UNIVERSITY [US/US]; Suite 906 111 Market Place Baltimore, MD 21202, US (AllExceptUS)
  • SNYDER, Solomon, H. [US/US]; US (UsOnly)
  • WOLOSKER, Herman [US/US]; US (UsOnly)
  • SHETH, Kevin [US/US]; US (UsOnly)
  • MASAAKI, Takahashi [US/US]; US (UsOnly)
  • MOTHET, Jean-Pierre [US/US]; US (UsOnly)
  • BRADY, Roscoe, O., Jr. [US/US]; US (UsOnly)
  • FERRIS, Christopher, D. [US/US]; US (UsOnly)
Inventors
  • SNYDER, Solomon, H.; US
  • WOLOSKER, Herman; US
  • SHETH, Kevin; US
  • MASAAKI, Takahashi; US
  • MOTHET, Jean-Pierre; US
  • BRADY, Roscoe, O., Jr.; US
  • FERRIS, Christopher, D.; US
Agents
  • KAGAN, Sarah, A. ; Banner & Witcoff, Ltd. 11th floor 1001 G Street, N.W. Washington, DC 20001-4597, US
Priority Data
60/116,33319.01.1999US
60/144,83921.07.1999US
60/145,95328.07.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MAMMALIAN SERINE RACEMASE
(FR) SERINE RACEMASE DE MAMMIFERE
Abstract
(EN)
High levels of D-serine occur in mammalian brain, where it appears to be an endogenous ligand of the 'glycine site' of NMDA receptors. We have purified from rat brain a soluble enzyme that catalyzes the direct racemization of L-serine to D-serine. Purified serine racemase has a molecular weight of 37 kDa and requires pyridoxal 5'-phosphate for its activity. The enzyme is highly selective toward L-serine, failing to racemize any other amino acid tested. We have also identified polynucleotide sequences which encode mammalian, including human, serine racemase. Compounds which modulate the activity of mammalian serine racemase are useful for treating conditions and diseases which involve overstimulation of NMDA receptors, such as stroke and various neurodegenerative diseases.
(FR)
La D-sérine se trouve à concentration élevé dans le cerveau des mammifères, où elle constitue un ligand endogène du «site glycine» des récepteurs NMDA. Nous avons purifié à partir du cerveau de rat une enzyme soluble qui catalyse la racémisation directe de la L-sérine en D-sérine. La sérine racémase purifiée a un poids moléculaire de 37 kDa et nécessite du pyridoxal 5'-phosphate pour son activité. L'enzyme est très sélective envers la L-sérine et ne peut réaliser la racémisation d'aucun autre acide aminé testé. Nous avons également identifié des séquences de polynucléotides qui codent pour la sérine racémase de mammifère, y compris de l'homme. Des composition qui modulent l'activité de la sérine racémase de mammifère permettent de traiter des pathologies et des maladies liées à une stimulation excessive des récepteurs NMDA, telles que les accidents vasculaires cérébraux et diverses maladies neurodégénératives.
Also published as
CN00805151.8
CZPV2001-2599
ININ/PCT/2001/00795/MUM
NO20013557
NZ513010
TR2001/02081
ZA200105621
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