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1. WO2000042432 - PURIFICATION PROCESS USING MAGNETIC PARTICLES

Publication Number WO/2000/042432
Publication Date 20.07.2000
International Application No. PCT/FI2000/000031
International Filing Date 18.01.2000
Chapter 2 Demand Filed 18.08.2000
IPC
B03C 1/01 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
1Magnetic separation
005Pretreatment specially adapted for magnetic separation
01by addition of magnetic adjuvants
C07K 1/22 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
16by chromatography
22Affinity chromatography or related techniques based upon selective absorption processes
C12N 7/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
02Recovery or purification
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
B03C 1/01
BPERFORMING OPERATIONS; TRANSPORTING
03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
1Magnetic separation
005Pretreatment specially adapted for magnetic separation
01by addition of magnetic adjuvants
C07K 1/22
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14Extraction; Separation; Purification
16by chromatography
22Affinity chromatography or related techniques based upon selective absorption processes
C12N 15/1013
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
1013by using magnetic beads
Applicants
  • THERMO LABSYSTEMS OY [FI/FI]; Sorvaajankatu 15 FIN-00810 Helsinki, FI (AllExceptUS)
  • SEPPÄNEN, Helena [FI/FI]; FI (UsOnly)
  • PALOMÄKI, Pekka [FI/FI]; FI (UsOnly)
  • TUUNANEN, Jukka [FI/FI]; FI (UsOnly)
  • KÄRMENIEMI, Timo [FI/FI]; FI (UsOnly)
Inventors
  • SEPPÄNEN, Helena; FI
  • PALOMÄKI, Pekka; FI
  • TUUNANEN, Jukka; FI
  • KÄRMENIEMI, Timo; FI
Agents
  • BERGGREN OY AB; P.O. Box 16 FIN-00101 Helsinki, FI
Priority Data
99008218.01.1999FI
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PURIFICATION PROCESS USING MAGNETIC PARTICLES
(FR) TECHNIQUE DE PURIFICATION FAISANT INTERVENIR DES PARTICULES MAGNETIQUES
Abstract
(EN)
The invention relates to a process for the purification of a substance with magnetic particles treated with a reagent which binds the particles. After a binding reaction in a first medium, the particles and the substance bound to them are separated and transferred to a second medium. According to the invention, a surface tension releasing agent is dispensed at least the mediums before the particles are separated from it. This promotes the complete collection of the particles.
(FR)
Cette invention a trait à une technique de purification d'une substance à l'aide de particules magnétiques traitées au moyen d'un réactif les liant. Après une réaction de liaison dans un premier milieu, les particules et la substance liée à celles-ci sont séparées et transférées vers un second milieu. Dans le cadre de cette invention un agent de libération de tension superficielle est introduit dans au moins l'un des milieux avant que les particules ne s'en séparent. Cette opération favorise la collecte de toutes les particules.
Also published as
NO20013541
US09646204
Latest bibliographic data on file with the International Bureau