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1. WO2000042418 - METHOD AND MATERIAL FOR RATIOMETRIC FLUORESCENT DETERMINATION OF ANALYTE CONCENTRATION

Publication Number WO/2000/042418
Publication Date 20.07.2000
International Application No. PCT/US2000/000800
International Filing Date 11.01.2000
IPC
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G01N 21/77 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
CPC
G01N 2021/6419
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6417Spectrofluorimetric devices
6419Excitation at two or more wavelengths
G01N 2021/6421
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6417Spectrofluorimetric devices
6421Measuring at two or more wavelengths
G01N 2021/6484
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
6484Optical fibres
G01N 2021/7786
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
7769Measurement method of reaction-produced change in sensor
7786Fluorescence
G01N 21/6428
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
G01N 21/7703
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77by observing the effect on a chemical indicator
7703using reagent-clad optical fibres or optical waveguides
Applicants
  • LIGHTSENSE CORPORATION [US/US]; Suite B 23452 Peralta Drive Laguna Hills, CA 92653, US
Inventors
  • RING, Lawrence, S.; US
  • ZOVAL, Jim, V.; US
Agents
  • KLEIN, Howard, J. ; Klein & Szekeres, LLP Suite 700 4199 Campus Drive Irvine, CA 92612, US
Priority Data
09/228,81911.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND MATERIAL FOR RATIOMETRIC FLUORESCENT DETERMINATION OF ANALYTE CONCENTRATION
(FR) PROCEDE ET APPAREIL DE DETERMINATION LOGOMETRIQUE PAR FLUORESCENCE DE LA CONCENTRATION D'UN ANALYTE
Abstract
(EN)
Optical sensors are used to measure the concentration of oxygen or other analytes in a sample. The radiometric method utilizes an indicator dye which is pyranthrene, flavanthrene or a suitable derivative dispersed or immobilized in an analyte permeable matrix. The matrix is excited with light at one or more wavelengths, or fluorescence emissions of the indicator are measured at one or more wavelengths, or both excitation and emission are performed and measured at multiple wavelengths. These indicator dyes exhibit different sensitivity to quenching by the analyte at different excitation wavelengths and also at different emission wavelengths. Each emission resulting from a different excitation is divided by the measured intensity of its excitation to compensate for illumination variations. The ratio of the resulting signals for each excitation is then used for determining the analyte concentration. Alternatively, the ratio of emissions at a single excitation wavelength are used in a similar manner.
(FR)
On utilise des capteurs optiques pour mesurer la concentration d'oxygène ou d'autres analytes dans un échantillon. Dans le procédé logométrique de la présente invention on utilise un colorant marqueur qui est du pyranthrène, du flavanthrène ou un dérivé approprié dispersé ou immobilisé dans une matrice perméable à l'analyte. La matrice est excitée avec de la lumière à une ou plusieurs longueurs d'onde, ou bien des émissions par fluorescence du marqueur sont mesurées à une ou plusieurs longueurs d'onde, ou bien encore il y a excitation et émission qui sont effectuées et mesurées à diverses longueurs d'onde. Ces colorants marqueurs présentent une sensibilité différente à l'extinction par l'analyte à des longueurs d'onde d'excitation différentes ainsi qu'à des longueurs d'onde d'émission différentes. Pour calculer la concentration de l'analyte, chaque émission résultant d'une excitation différente est divisée par l'intensité mesurée de sa propre excitation pour compenser les variations d'illumination. Le rapport des signaux résultants pour chaque excitation est ensuite utilisé pour déterminer la concentration en analyte ; ou bien c'est le rapport des émissions à une longueur d'onde d'excitation unique qui est utilisé d'une manière similaire.
Also published as
Latest bibliographic data on file with the International Bureau