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1. WO2000042215 - METHOD AND POLYNUCLEOTIDES FOR DETERMINING TRANSLATIONAL EFFICIENCY OF A CODON

Publication Number WO/2000/042215
Publication Date 20.07.2000
International Application No. PCT/AU2000/000008
International Filing Date 07.01.2000
Chapter 2 Demand Filed 28.07.2000
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/67
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12Q 1/6897
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6897involving reporter genes operably linked to promoters
Applicants
  • THE UNIVERSITY OF QUEENSLAND [AU/AU]; St. Lucia, Queensland 4072, AU (AllExceptUS)
  • SUN, Xiao-Yi [AU/AU]; AU (UsOnly)
  • FRAZER, Ian, Hector [AU/AU]; AU (UsOnly)
Inventors
  • FRAZER, Ian, Hector; AU
  • ZHOU, Jian; null
Agents
  • ARGAET, Victor, P. ; Davies Collison Cave 1 Little Collins Street Melbourne, VIC 3000, AU
Priority Data
PP 807808.01.1999AU
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND POLYNUCLEOTIDES FOR DETERMINING TRANSLATIONAL EFFICIENCY OF A CODON
(FR) POLYNUCLEOTIDES ET PROCEDE PERMETTANT DE DETERMINER L'EFFICACITE DE LA TRADUCTION D'UN CODON
Abstract
(EN)
A method is disclosed for determining the translational efficiency of an individual codon in a cell. The method comprises introducing into the cell a synthetic construct comprising a reporter polynucleotide fused in frame with a tandem repeat of said individual codon, wherein said reporter polynucleotide encodes a reporter protein, and wherein said synthetic construct is operably linked to a regulatory polynucleotide and measuring expression of said reporter protein in said cell to determine the translational efficiency of said codon.
(FR)
La présente invention concerne un procédé permettant de déterminer l'efficacité de la traduction d'un codon individuel dans une cellule. Ce procédé consiste à introduire dans la cellule une construction synthétique comprenant un polynucléotide marqueur fusionné dans un cadre avec une séquence nucléotidique répétée en tandem dudit codon individuel. Le polynucléotide marqueur code une protéine marqueur et ladite construction synthétique est liée fonctionnellement à un polynucléotide régulateur. Le procédé consiste ensuite à mesurer l'expression de ladite protéine marqueur dans la cellule afin de déterminer l'efficacité de la traduction du codon.
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