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1. WO2000042210 - MICROSATELLITE DNA MARKERS AND USES THEREOF

Publication Number WO/2000/042210
Publication Date 20.07.2000
International Application No. PCT/US2000/000325
International Filing Date 06.01.2000
Chapter 2 Demand Filed 12.07.2000
IPC
C12P 19/34 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C07H 21/02 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02with ribosyl as saccharide radical
CPC
C12Q 1/6876
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q 1/6895
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
6895for plants, fungi or algae
C12Q 2600/156
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
156Polymorphic or mutational markers
Applicants
  • INTERNATIONAL PAPER COMPANY [US/US]; Two Manhattanville Road Purchase, NY 10577, US
  • UNITED STATES OF AMERICA, as represented by THE SECRETARY OF AGRICULTURE [US/US]; Washington, DC 20250, US
Inventors
  • ECHT, Craig, S.; NZ
  • NELSON, C., Dana; US
Agents
  • ZITRON, Anne, E. ; Darby & Darby P.C. 805 third Avenue New York, NY 10022-7513, US
  • ZIELINSKI Walt Thomas DARBY & DARBY P.C.; 805 Third Avenue New York, New York 10022-7513, US
Priority Data
09/232,78519.01.1999US
09/232,88415.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MICROSATELLITE DNA MARKERS AND USES THEREOF
(FR) MARQUEURS D'ADN MICROSATELLITES ET LEURS UTILISATIONS
Abstract
(EN)
Microsatellite, simple sequence repeat (SSR), markers have much potential for enhancing genome mapping and genotype identification research in forest genetics and tree breeding. SSR markers were developed by isolating and sequencing 1539 loblolly pine SSR clones for 11 SSR motifs. After screening out redundancy among the sequences, 566 oligonucleotide PCR primer pairs flanking the SRRs were synthesized and evaluated for their ability to amplify genomic DNA from loblolly pine. The three SSR motifs that yielded the highest proportion of informative markers from sequenced clones were (AC)n, (AAAT)n, and (AAAC)n. Eighteen polymorphic tri-a nd tetranucleotide SSR loci were genotyped in 20 loblolly pine trees using automated fluorescent marker analysis. The average number of alleles per locus observed was 6.4, and the average polymorphism information content (PIC) was 0.547. Subsets of the 566 primer pairs were evaluated for their ability to amplify DNA from six other pine species, and 54 primer pairs amplified markers thatwere polymorphic among the species. The present invention also concerns the methods of using the identified SSR loci as genetic markers.
(FR)
L'invention porte sur des marqueurs microsatellites, à répétition de séquence (SSR), qui possèdent un fort potentiel pour améliorer la cartographie génomique et la recherche d'identification de génotype dans la génétique forestière et l'arboriculture. Les marqueurs SSR ont été développés par isolement et séquençage des clones SSR du pin négresse 1539 pour les motifs structuraux SSR 11. Après avoir recherché les redondances dans les séquences, 566 paires d'amorces oligonucléotidiques de la PCR sur le flanc des SRR ont été synthétisées et évaluées pour leur capacité à amplifier l'ADN génomique à partir du pin négresse. Les trois motifs structuraux des SSR qui ont produit la plus importante proportion de marqueurs informatifs à partir des clones séquencés étaient (AC)n, (AAAT)n, et (AAAC)n. Dix-huit loci SSR polymorphes tri- et té tranucléotidiques ont été génotypés dans 20 pins négresse par analyse automatique des marqueurs fluorescents. Le nombre moyen des allèles par locus observé était de 6,4, et le contenu moyen des informations sur le polymorphisme (PIC) était de 0,547. Les sous-ensembles des 566 amorces primaires ont été évalués pour leur capacité à amplifier l'ADN à partir de six espèces d'autres pins, et 54 marqueurs amplifiés par les paires d'amorces qui étaient des polymorphes parmi les espèces. Cette invention porte également sur des procédés d'utilisation des loci SSR identifiés comme marqueurs génétiques.
Also published as
NZ512941
Latest bibliographic data on file with the International Bureau