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1. WO2000042207 - SOYBEAN TRANSFORMATION METHOD

Publication Number WO/2000/042207
Publication Date 20.07.2000
International Application No. PCT/US2000/000791
International Filing Date 12.01.2000
Chapter 2 Demand Filed 08.08.2000
IPC
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C12N 15/8205
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8202by biological means, e.g. cell mediated or natural vector
8205Agrobacterium mediated transformation
Applicants
  • MONSANTO TECHNOLOGY LLC [US/US]; 800 N. Lindbergh Avenue St. Louis, MO 63167, US
Inventors
  • WILLIAMS, Edward, J.; US
  • EMLER, Carol, A.; US
  • JULSON, Lori, S.; US
  • MARTINELL, Brian, J.; US
  • MCCABE, Dennis, E.; US
  • HUANG, Yong; US
Agents
  • KAMMERER, Patricia, A.; Arnold White & Durkee 750 Bering Drive Houston, TX 77057-2198, US
  • BOSCH, Henry A. Monsanto Services International SA/NV; Avenue de Tervuren 270-272 1150 Brussels Belgium, BE
Priority Data
60/115,83314.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SOYBEAN TRANSFORMATION METHOD
(FR) PROCEDE DE TRANSFORMATION DE SOJA
Abstract
(EN)
A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.
(FR)
L'invention concerne un procédé de transformation génétique des cellules germinales du soja par l'intermédiaire de Agrobacterium. Ce procédé repose sur l'introduction par l'intermédiaire de Agrobacterium de gènes dans les cellules individuelle du méristème du soja fraîchement germé, ces cellules pouvant être activées de manière à former directement des pousses donnant des plantes transgéniques. Ce procédé est rapide et efficace et ne comprend pas de culture de tissus en phase de cal.
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