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1. WO2000042203 - METHODS FOR PRODUCING POLYPEPTIDES IN CYCLOHEXADEPSIPEPTIDE-DEFICIENT CELLS

Publication Number WO/2000/042203
Publication Date 20.07.2000
International Application No. PCT/US2000/000913
International Filing Date 13.01.2000
Chapter 2 Demand Filed 08.08.2000
IPC
C12N 9/88 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88Lyases (4.)
C12N 15/80 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
C12P 21/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
CPC
C12N 15/80
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
C12N 9/88
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
C12P 21/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
C12R 1/77
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
645using fungi
77Fusarium
Applicants
  • NOVOZYMES BIOTECH, INC. [US/US]; 1445 Drew Avenue Davis, CA 95616, US (AllExceptUS)
  • BERKA, Randy, M. [--/US]; US (UsOnly)
  • REY, Michael, W. [--/US]; US (UsOnly)
  • YODER, Wendy, T. [--/US]; US (UsOnly)
Inventors
  • BERKA, Randy, M.; US
  • REY, Michael, W.; US
  • YODER, Wendy, T.; US
Agents
  • STARNES, Robert, L.; Novozymes Biotech, Inc. 1445 Drew Avenue Davis, CA 95616, US
Priority Data
09/229,86213.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR PRODUCING POLYPEPTIDES IN CYCLOHEXADEPSIPEPTIDE-DEFICIENT CELLS
(FR) PROCEDE DE PRODUCTION DE POLYPEPTIDES DANS DES CELLULES DEFICITAIRES EN CYCLOHEXADEPSIPEPTIDE
Abstract
(EN)
The present invention relates to methods for producing a heterologous polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the heterologous polypeptide, wherein (i) the mutant cell comprises a nucleic acid sequence encoding the heterologous polypeptide and (ii) the mutant produces less of the cyclohexadepsipeptide than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the heterologous polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated cyclohexadepsipeptide synthetases and isolated nucleic acid sequences encoding the cyclohexadepsipeptide synthetases. The present invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the cyclohexadepsipeptide synthetases. The present invention further relates to cyclohexadepsipeptides produced by the cyclohexadepsipeptide synthetases.
(FR)
Cette invention porte sur un procédé permettant la production d'un polypeptide hétérologue. Ce procédé consiste (a) à cultiver un mutant d'une cellule fongique mère filamenteuse dans des conditions propres à la production du polypeptide hétérologue. En l'occurrence, (i) la cellule mutante comprend une séquence d'acide nucléique codant le polypeptide hétérologue, et (ii) le mutant produit moins de cyclohexadepsipeptide que la cellule fongique mère filamenteuse cultivée dans les mêmes conditions. Le procédé consiste ensuite (b) à isoler du milieu de culture le polypeptide hétérologue. L'invention concerne également des mutants de cellules fongiques filamenteuses et des procédés d'obtention de ces cellules mutantes. L'invention concerne également des synthases de cyclohexadepsipeptide isolées et des séquences d'acides nucléiques isolées codant les synthases de cyclohexadepsipeptide. L'invention concerne aussi des constructions d'acide nucléique, des vecteurs et des cellules hôtes comprenant ces séquences d'acides nucléiques ainsi que des procédés de production des synthases de cyclohexadepsipeptide. L'invention concerne enfin des cyclohexadepsipeptides produites par des synthases de cyclohexadepsipeptide.
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