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1. WO2000042177 - METHOD FOR ISOLATING DNA FROM BIOLOGICAL MATERIALS

Publication Number WO/2000/042177
Publication Date 20.07.2000
International Application No. PCT/EP2000/000052
International Filing Date 05.01.2000
Chapter 2 Demand Filed 21.06.2000
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/1003
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applicants
  • MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V. [DE/DE]; Hofgartenstrasse 2 D-80539 München, DE (AT, BE, CA, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, JP, LU, MC, NL, PT, SE)
  • QIAGEN GMBH [DE/DE]; Max-Volmer-Strasse 4 D-40724 Hilden, DE (AT, BE, CA, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, JP, LU, MC, NL, PT, SE)
  • MÜLLER, Oliver [DE/DE]; DE (UsOnly)
  • SPRENGER-HAUSSELS, Markus [DE/DE]; DE (UsOnly)
  • BASTIAN, Helge [DE/DE]; DE (UsOnly)
  • VOLLERT, Stefanie [DE/DE]; DE (UsOnly)
Inventors
  • MÜLLER, Oliver; DE
  • SPRENGER-HAUSSELS, Markus; DE
  • BASTIAN, Helge; DE
  • VOLLERT, Stefanie; DE
Agents
  • WEICKMANN, H. ; Kopernikusstrasse 9 D-81679 München, DE
Priority Data
199 00 638.511.01.1999DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) METHODE ZUR ISOLIERUNG VON DNA AUS BIOLOGISCHEN MATERIALIEN
(EN) METHOD FOR ISOLATING DNA FROM BIOLOGICAL MATERIALS
(FR) PROCEDE POUR ISOLER DE L'ADN CONTENU DANS DES MATIERES BIOLOGIQUES
Abstract
(DE)
Die Erfindung betrifft ein Verfahren zur Stabilisierung, Reinigung oder/und Isolierung von Nukleinsäuren aus biologischen Materialien, insbesondere Stuhlproben, die Verunreinigungen und Störsubstanzen enthalten können. Weiterhin wird ein für die Durchführung des erfindungsgemäßen Verfahrens geeigneter Reagenzienkit beschrieben.
(EN)
The invention relates to a method for stabilizing, purifying and/or isolating nucleic acids from biological materials, especially from stool samples, which can contain impurities and interfering substances. The invention also relates to a reagent kit which is suited for carrying out the inventive method.
(FR)
L'invention concerne un procédé permettant de stabiliser, de nettoyer et/ou d'isoler des acides nucléiques contenus dans des matières biologiques, notamment des échantillons de selles prélevés, susceptibles de contenir des impuretés et des substances parasites. L'invention concerne en outre un coffret de réactifs utilisé pour mettre ledit procédé en oeuvre.
Also published as
EP2008014519
Latest bibliographic data on file with the International Bureau