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1. WO2000042167 - EUKARYOTIC CELL-BASED SYSTEM FOR IDENTIFYING GENE MODULATORS

Publication Number WO/2000/042167
Publication Date 20.07.2000
International Application No. PCT/AU1999/001131
International Filing Date 21.12.1999
Chapter 2 Demand Filed 02.08.2000
IPC
C12N 15/81 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/815
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
815for yeasts other than Saccharomyces
C12Q 1/6897
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6897involving reporter genes operably linked to promoters
Applicants
  • JOHNSON & JOHNSON RESEARCH PTY. LIMITED [AU/AU]; Australian Technology Park Level 4 1 Central Avenue Eveleigh, NSW 1430, AU (AllExceptUS)
  • ARNDT, Gregory, Martin [CA/AU]; AU (UsOnly)
  • ATKINS, David, George [AU/AU]; AU (UsOnly)
Inventors
  • ARNDT, Gregory, Martin; AU
  • ATKINS, David, George; AU
Agents
  • BALDWIN SHELSTON WATERS; 60 Margaret Street Sydney, NSW 2000, AU
Priority Data
10094/9908.01.1999AU
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) EUKARYOTIC CELL-BASED SYSTEM FOR IDENTIFYING GENE MODULATORS
(FR) SYSTEME PERMETTANT D'IDENTIFIER DES MODULATEURS DE GENE ARTICULE AUTOUR DE CELLULES EUCARYOTES
Abstract
(EN)
The present invention is concerned with nucleic acid fusion constructs capable of being expressed when introduced into $i(Schizosaccharomyces pombe). The fusion constructs consist essentially of a target nucleic acid sequence and a reporter nucleic acid sequence, wherein the target and reporter sequences are situated with respect to each other so that the expression of the reporter sequence is modulated by any molecule which also modulates the expression of the target sequence. The expression of the reporter sequence confers on $i(Schizosaccharomyces pombe) a detectable phenotype. The invention is also concerned with methods for determining whether, or to what degree, a molecule specifically modulates the expression of a target nucleic acid sequence in a eukaryotic cell.
(FR)
Cette invention a trait à des produits de recombinaison de fusion d'acide nucléique pouvant être exprimés une fois introduits dans$i( Schizosaccharomyces pombe). Ces produits de recombinaison de fusion sont principalement constitués d'une séquence nucléotidique cible et d'une séquence nucléotidique reporter, ces séquences, cible et reporter, étant situées l'une par rapport à l'autre de manière que l'expression de la séquence reporter soit modulée par toute molécule modulant également l'expression de la séquence cible. L'expression de la séquence reporter confère un phénotype décelable à $i(Schizosaccharomyces pombe). Cette invention porte également sur des techniques permettant de déterminer si, ou à quel degré, une molécule module de manière spécifique une séquence nucléotidique cible dans une cellule eucaryote.
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