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1. WO2000042048 - A REAGENT SYSTEM AND METHOD FOR INCREASING THE LUMINESCENCE OF LANTHANIDE(III) MACROCYCLIC COMPLEXES

Publication Number WO/2000/042048
Publication Date 20.07.2000
International Application No. PCT/US2000/001211
International Filing Date 18.01.2000
Chapter 2 Demand Filed 18.08.2000
IPC
C07D 471/18 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
DHETEROCYCLIC COMPOUNDS
471Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/-C07D463/251
12in which the condensed system contains three hetero rings
18Bridged systems
G01N 33/58 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
C07D 471/18
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
DHETEROCYCLIC COMPOUNDS
471Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
12in which the condensed system contains three hetero rings
18Bridged systems
G01N 33/582
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
582with fluorescent label
Y10S 435/96
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
435Chemistry: molecular biology and microbiology
96Immunohistochemical assay
Y10S 435/968
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
435Chemistry: molecular biology and microbiology
968High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
Applicants
  • LEIF, Robert, C. [US/US]; US
  • VALLARINO, Lidia [US/US]; US
Inventors
  • LEIF, Robert, C.; US
  • VALLARINO, Lidia; US
Agents
  • SCHWARTZ, Robert, M.; Robert M. Schwartz, P.A. Suite 1125 169 E. Flagler Street Miami, FL 33131-1205, US
Priority Data
60/116,31619.01.1999US
null18.01.2000US (Considered void 07.08.2000)
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) A REAGENT SYSTEM AND METHOD FOR INCREASING THE LUMINESCENCE OF LANTHANIDE(III) MACROCYCLIC COMPLEXES
(FR) SYSTEME REACTIF ET PROCEDE D'AUGMENTATION DE LA LUMINESCENCE DE COMPLEXES MACROCYCLIQUES LANTHANIDIQUES (III)
Abstract
(EN)
Disclosed are a spectrofluorimetrically detectable luminescent composition and processes for enhancing the luminescence of one or more lanthanide-containing macrocycles. The luminescent composition comprises a micelle-producing amount of at least one surfactant, at least one energy transfer acceptor lanthanide element macrocycle compound having an emission spectrum peak in the range from 500 to 950 nanometers, and a luminescence-enhancing amount of at least one energy transfer donor compound of yttrium or a 3-valent lanthanide element having atomic number 59-71, provided that the lanthanide element of said macrocycle compound and the lanthanide element of said energy transfer donor compound are not identical. The addition of gadolinium(III) in the presence of other solutes to both the prototype and the difunctionalized europium, samarium, and terbium macrocyclic complexes, which were taught in our U.S. patents #5,373,093 and #5,696,240, enhances their luminescence. Similar enhancements of luminescence also results for the mono-functionalized europium, samarium, and terbium macrocyclic complexes, which were taught in our U.S. patent #5,696,240. The enhanced luminescence afforded by the composition enables the detection and/or quantitation of many analytes in low concentrations without the use of expensive, complicated time-gated detection systems.
(FR)
La présente invention concerne une composition luminescente détectable par spectrofluorimétrie ainsi que des procédés d'augmentation de la luminescence d'un ou de plusieurs macrocycles à base lanthanidique. Cette composition luminescente comprend une quantité de production micellaire d'au moins un tensioactif, au moins un composé macrocyclique d'élément lanthanidique accepteur de transfert énergétique présentant un pic de spectre d'émission compris entre 500 et 950 nanomètres, et un niveau d'augmentation de luminescence d'au moins un composé de yttrium donneur de transfert énergétique ou un élément lanthanidique trivalent ayant un nombre atomique compris entre 59 et 71, sous réserve que l'élément lanthanidique du composé macrocyclique et l'élément lanthanidique du composé donneur de transfert énergétique ne soient pas identiques. L'ajout de gadolinium (III) en présence d'autres solutés à la fois dans les complexes macrocycliques prototypes et défonctionnalisés comme l'europium, le samarium, et le terbium, décrits dans les brevets US N°5,373,093 et 5,696,240, à pour effet d'augmenter leur luminescence. En outre, on peut obtenir une luminescence similaire avec les complexes macrocycliques mono-fonctionnalisés comme l'europium, le samarium, et le terbium, décrits dans le brevet US. N°5,696,240. La luminescence augmentée obtenue grâce à cette composition permet de détecter et/ou de quantifier plusieurs analytes dans de faibles concentrations sans avoir à utiliser des systèmes de détection à résolution temporelle compliqués et chers.
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