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1. WO2000041561 - BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE

Publication Number WO/2000/041561
Publication Date 20.07.2000
International Application No. PCT/JP2000/000132
International Filing Date 13.01.2000
Chapter 2 Demand Filed 27.06.2000
IPC
C07K 14/705 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
C12N 15/85 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
CPC
A01K 2217/075
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2217Genetically modified animals
07Animals genetically altered by homologous recombination
075inducing loss of function, i.e. knock out
A01K 2227/105
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2227Animals characterised by species
10Mammal
105Murine
A01K 2267/03
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2267Animals characterised by purpose
03Animal model, e.g. for test or diseases
A01K 2267/0337
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2267Animals characterised by purpose
03Animal model, e.g. for test or diseases
0337Animal models for infectious diseases
A01K 67/0276
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67Rearing or breeding animals, not otherwise provided for; New breeds of animals
027New breeds of vertebrates
0275Genetically modified vertebrates, e.g. transgenic
0276Knockout animals
C07K 14/705
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
Applicants
  • JAPAN SCIENCE AND TECHNOLOGY CORPORATION [JP/JP]; 1-8, Honcho 4-chome Kawaguchi-shi, Saitama 332-0012, JP (AllExceptUS)
  • AKIRA, Shizuo [JP/JP]; JP (UsOnly)
  • TAKEUCHI, Osamu [JP/JP]; JP (UsOnly)
  • TAKEDA, Kiyoshi [JP/JP]; JP (UsOnly)
Inventors
  • AKIRA, Shizuo; JP
  • TAKEUCHI, Osamu; JP
  • TAKEDA, Kiyoshi; JP
Agents
  • HIROTA, Masanori; Room 502, Akasakaaoi Bldg. No.11, 8-11 Akasaka 2-chome Minato-ku Tokyo 107-0052, JP
Priority Data
11/22828212.08.1999JP
11/30923829.10.1999JP
11/736514.01.1999JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE
(FR) MODELE MURIN NE REPONDANT PAS AUX COMPOSANTS CELLULAIRES BACTERIENS
Abstract
(EN)
A knockout mouse unresponsive to peptideglycans, lipoproteins, lipopeptides, etc. which are useful in clarifying the role of each member of the TLR family (in particular, TLR2 and MyD88) in the signal transduction due to stimulation with bacterial cell components $i(in vivo). This knockout mouse is prepared by the homologous recombination method with the use of a targeting vector constructed by substituting the whole gene fragment or a part thereof of the exon site containing the intracellular region of TLR2 or MyD88 gene by a plasmid having poly(A) signal and a marker gene.
(FR)
Souris knockout ne répondant pas aux peptidoglycanes, lipoprotéines, lipopeptides, etc., qui est utile pour clarifier le rôle de chaque membre de la famille TLR (notamment TLR2 et MyD88) dans la traduction des signaux due à la stimulation par des composants cellulaires bactériens $i(in vivo). Cette souris knockout est préparée par une technique de recombinaison homologue au moyen d'un vecteur de ciblage. Pour construire ledit vecteur, on remplace le gène entier ou une partie dudit gène, au niveau du site d'exon contenant la région intracellulaire de TLR2 ou MyD88, par un plasmide comportant un signal poly(A) et un gène marqueur.
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