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1. WO2000041549 - BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION

Publication Number WO/2000/041549
Publication Date 20.07.2000
International Application No. PCT/US2000/000972
International Filing Date 14.01.2000
Chapter 2 Demand Filed 08.08.2000
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6816
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
C12Q 1/6818
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6818involving interaction of two or more labels, e.g. resonant energy transfer
Applicants
  • PE CORPORATION (NY) [US/US]; 850 Lincoln Centre Drive Foster City, CA 94404, US
Inventors
  • LIVAK, Kenneth, J.; US
  • EGHOLM, Michael; US
  • HUNKAPILLER, Michael, W.; US
Agents
  • ANDRUS, Alex ; PE Corporation 850 Lincoln Centre Drive Foster City, CA 94404, US
  • VOSSIUS, Volker et al.; Holbeinstr. 5, D-81679, DE
Priority Data
09/232,00015.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BINARY PROBE AND CLAMP COMPOSITION AND METHODS FOR TARGET HYBRIDIZATION DETECTION
(FR) COMPOSITION BINAIRE SONDE/PINCE, ET METHODES DE DETECTION D'HYBRIDATIONS CIBLE
Abstract
(EN)
Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.
(FR)
L'invention concerne des compositions binaires sondes/pinces permettant de mettre en oeuvre des méthodes de détection d'hybridations cible. Lorsque la sonde est un substrat destiné au clivage des exonucléases, la composition permet une quantification et une détection de produits d'amplification PCR, par des mesures en temps réel et des analyses des points terminaux. Lorsque la sonde est une amorce d'amplification, la composition permet de mettre en oeuvre une méthode améliorée de marquage et de détection des produits d'amplification PCR. Les sondes et les pinces précitées peuvent être marquées à l'aide de colorants fluorescents, d'extincteurs de luminescence, de fractions de stabilisation de l'hybridation, de colorants chimiluminescents, et de ligands d'affinité. Les pinces peuvent être des analogues d'acide nucléique, tels que 2-aminoéthylglycine (PNA).
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