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1. WO2000040973 - ASSAY FOR HOMOCYSTEINE

Publication Number WO/2000/040973
Publication Date 13.07.2000
International Application No. PCT/GB1999/004442
International Filing Date 30.12.1999
Chapter 2 Demand Filed 04.08.2000
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
Applicants
  • AXIS-SHIELD ASA [NO/NO]; Ulvenveien 87 P.O. Box 206 Økern N-0510 Oslo, NO (AllExceptUS)
  • COCKBAIN, Julian [GB/GB]; GB (GB)
  • FRANTZEN, Frank [NO/NO]; NO (UsOnly)
Inventors
  • FRANTZEN, Frank; NO
Agents
  • COCKBAIN, Julian ; Frank B. Dehn & Co. 179 Queen Victoria Street London EC4V 4EL, GB
Priority Data
9900159.605.01.1999GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ASSAY FOR HOMOCYSTEINE
(FR) DOSAGE D'HOMOCYSTEINE
Abstract
(EN)
The invention provides a method for assaying homocysteine in a sample, said method comprising: contacting said sample with two or three stable aqueous reagents containing a polyhapten, a homocysteine converting enzyme, a primary antibody capable of binding to said polyhapten whereby to produce a complex, and if desired one or more of a co-substrate for said homocysteine converting enzyme, a reducing agent, a further enzyme capable of converting said co-substrate or a conversion product of said homocysteine converting enzyme, and a second antibody capable of binding to said complex, said primary antibody also being capable of binding to said co-substrate or a conversion product of one of said enzymes whereby the quantity of said complex produced is indicative of the content of homocysteine in said sample; and photometrically detecting said complex.
(FR)
Cette invention concerne une méthode de dosage d'homocystéine dans un échantillon, qui consiste à : mettre ledit échantillon en contact avec deux ou trois réactifs aqueux stables renfermant un polyhaptène, un enzyme de conversion de l'homocystéine, un anticorps primaire capable de se lier audit polyhaptène pour produire un complexe et, le cas échéant, un ou plusieurs co-substrats pour ledit enzyme de conversion de l'homosystéine, un agent réducteur, un autre enzyme capable de convertir ledit co-substrat et un second anticorps capable de se lier audit complexe, l'anticorps primaire pouvant également se lier audit co-substrat ou à un produit de conversion de l'un desdits enzymes, la quantité dudit complexe produit renseignant sur la teneur de l'échantillon en homocystéine ; et détecter ledit complexe par photométrie.
Also published as
US09869060
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