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1. WO2000040967 - METHOD AND DEVICE FOR DIAGNOSING ALLERGY SYMPTOMS

Publication Number WO/2000/040967
Publication Date 13.07.2000
International Application No. PCT/DE2000/000001
International Filing Date 03.01.2000
Chapter 2 Demand Filed 04.08.2000
IPC
G01N 33/543 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
CPC
B01J 2219/00605
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
B01J 2219/00612
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
00612the surface being inorganic
B01J 2219/00617
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
00614Delimitation of the attachment areas
00617by chemical means
B01J 2219/0063
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
00623Immobilisation or binding
0063Other, e.g. van der Waals forces, hydrogen bonding
B01J 2219/00637
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00605the compounds being directly bound or immobilised to solid supports
00632Introduction of reactive groups to the surface
00637by coating it with another layer
B01J 2219/00659
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00583Features relative to the processes being carried out
00603Making arrays on substantially continuous surfaces
00659Two-dimensional arrays
Applicants
  • SEILER, Jürgen [DE/DE]; DE (AllExceptUS)
  • MESSER, Horst [DE/DE]; DE
Inventors
  • MESSER, Horst; DE
Agents
  • FIEDLER, Jürgen; Fiedler & Ostermann Heiligenbreite 7 D-37176 Nörten-Hardenberg, DE
Priority Data
199 00 119.706.01.1999DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) VERFAHREN UND VORRICHTUNG ZUR DIAGNOSE ALLERGISCHER SYMPTOME
(EN) METHOD AND DEVICE FOR DIAGNOSING ALLERGY SYMPTOMS
(FR) PROCEDE ET DISPOSITIF POUR LE DIAGNOSTIC DE SYMPTOMES ALLERGIQUES
Abstract
(DE)
Verfahren und Vorrichtung zur Diagnose allergischer Symptome durch Nachweis von in vitro Immunreaktionen, bei dem auf einem Träger ein Bindungsprotein aufgebracht wird, auf dem Antikörper eines zu untersuchenden Patienten durch Ankoppeln an das Bindungsprotein immobilisiert und anschließend einer Antigen-Lösung ausgesetzt werden, die für den Patienten allergieauslösende Antigene enthält, wobei der Träger als reflektierender fester Träger ausgebildet ist, auf dem das Bindungsprotein in einer monomolekularen, oberflächenaktiven Schicht aufgebracht wird, und wobei nach einer Inkubationszeit ein durch Immunreaktion zwischen dem Antikörper und dem Antigen verursachter Schichtdickenzuwachs der Protein-Antikörper-Schicht ellipsometrisch festgestellt wird.
(EN)
The invention relates to a method and device for diagnosing allergy symptoms by detecting in vitro immunoreactions. According to the inventive method, a binding protein is applied to a substrate on which antibodies of a patient that is to be examined are immobilized by coupling to the binding protein, and are subsequently exposed to an antigen solution containing the antigens which trigger the allergy for the patient. The substrate is configured as a reflective solid substrate on which the binding protein is applied in a monomolecular, surface-active layer. After an incubation time, a growth in layer thickness of the protein antibody layer is ellipsometrically determined, said growth being caused by an immunoreaction between the antibody and the antigen.
(FR)
L'invention concerne un procédé pour le diagnostic de symptômes allergiques par mise en évidence de réactions immunitaires in vitro. Dans le procédé selon l'invention, on applique sur un substrat une protéine de liaison sur laquelle des anticorps d'un patient à examiner sont immobilisés par accouplement avec la protéine de liaison puis soumis à une solution antigène contenant des antigènes déclencheurs d'allergie pour le patient. Le substrat est réalisé sous forme de substrat fixe réfléchissant sur lequel la protéine de liaison est appliquée en une couche monomoléculaire à surface active et on constate par mesure ellipsométrique après un temps d'incubation une croissance de l'épaisseur de la couche protéine-anticorps due à une réaction immunitaire entre l'anticorps et l'antigène. L'invention concerne également un dispositif pour le diagnostic de symptômes allergiques par mise en évidence de réactions immunitaires in vitro, dans lequel une base de détection est réalisée sous forme d'un substrat fixe réfléchissant sur lequel on applique, en îlots sous forme de matrice, une couche monomoléculaire d'une protéine de liaison à surface active à laquelle peuvent se relier des anticorps spécifiques du patient pouvant réagir avec des antigènes contenus dans une solution.
Also published as
US09889001
Latest bibliographic data on file with the International Bureau