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1. WO2000040966 - METHOD AND KIT FOR EXTRACTING PRION PROTEIN

Publication Number WO/2000/040966
Publication Date 13.07.2000
International Application No. PCT/US2000/000457
International Filing Date 07.01.2000
Chapter 2 Demand Filed 07.08.2000
IPC
C07K 14/47 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
CPC
C07K 14/47
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
Y10S 435/962
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
435Chemistry: molecular biology and microbiology
962Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
Applicants
  • THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF AGRICULTURE [US/US]; 1400 Independence Avenue SW Washington, DC 20250-0302, US
  • ALPERT, Andrew, J. [US/US]; US
Inventors
  • ALPERT, Andrew, J.; US
  • SCHMERR, Mary, Jo; US
Agents
  • PENDORF, Stephan, A. ; Pendorf & Cutliff P.O. Box 20445 Tampa, FL 33622-0445, US
Priority Data
09/420,85019.10.1999US
60/115,27208.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND KIT FOR EXTRACTING PRION PROTEIN
(FR) PROCEDE ET KIT D'EXTRACTION DE PROTEINE PRION
Abstract
(EN)
A method for extracting prion protein from a biological material, e.g., an animal tissue or product. In a specific example, abnormal prion protein is extracted from homogenized sheep brain with hexafluoro-2-propanol. The hexafluoro-2-propanol is separated from the aqueous brain preparation by increasing the ionic strength of the aqueous solution. Prion protein in the organic extract can be further purified, or the extract can be tested, e.g., by immunoassay, for the presence of prion protein, and more particularly abnormal prion protein. The extraction process permits testing for the presence of abnormal prion protein, e.g., for diagnosis of transmissible spongiform encephalopathies (TSE). The figure shows chromatogram from HILIC of I-prion protein as detected by radioactivity which is represented by open circles and by absorbance at 280 nm as represented by solid line in the graph.
(FR)
L'invention concerne un procédé d'extraction de protéine prion contenue dans une substance biologique telle qu'un tissu animal ou un produit animal. Dans un exemple spécifique, une protéine prion anormale est extraite d'un cerveau de brebis homogénéisé avec de l'hexafluoro-2-propanol, que l'on sépare de la préparation aqueuse à base de cerveau en augmentant la force ionique de la solution. Les protéines prions présentes dans cet extrait organique peuvent être encore purifiées, mais il est également possible d'analyser cet extrait, notamment par dosage immunologique, afin de détecter la présence de protéines prions et plus particulièrement de protéines prions anormales. Le processus d'extraction permet d'analyser la présence de protéine prion anormale, notamment en vue de diagnostiquer des encéphalopathies spongiformes transmissibles (TSE). La figure montre un chromatogramme obtenu à partir de la chromatographie par interactions hydrophiles (HILIC) de protéine prion-I détectée par la radioactivité, représentée par des cercles, et par l'absorbance à 280 nm, représentée en trait plein sur le graphique.
Also published as
BG105791
CZPV2001-2469
GE6037/1
ININ/PCT/2001/657/KOL
NO20013193
SK200100949
TR2001/01935
UZ20010673
YUP-480/01
ZA200105304
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