Processing

Please wait...

PATENTSCOPE will be unavailable a few hours for maintenance reason on Sunday 05.04.2020 at 10:00 AM CEST
Settings

Settings

1. WO2000040754 - DNA OLIGONUCLEOTIDE WHICH IS SPECIFIC FOR MELOIDOGYNE SPECIES, DNA VECTOR AND HOST CELL CONTAINING THE OLIGONUCLEOTIDE, USE AND KIT

Publication Number WO/2000/040754
Publication Date 13.07.2000
International Application No. PCT/NL1999/000812
International Filing Date 28.12.1999
Chapter 2 Demand Filed 11.07.2000
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6888
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
C12Q 2600/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
16Primer sets for multiplex assays
Applicants
  • STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK (IPO-DL O) [NL/NL]; Bornsesteeg 53 NL-6708 PD Wageningen, NL (AllExceptUS)
  • ZIJLSTRA, Carolien [NL/NL]; NL (UsOnly)
Inventors
  • ZIJLSTRA, Carolien; NL
Agents
  • VAN KAN, J., J., H. ; Algemeen Octrooibureau World Trade Center Pastoor Petersstraat 160 NL-5612 LV Eindhoven, NL
Priority Data
101091730.12.1998NL
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DNA OLIGONUCLEOTIDE WHICH IS SPECIFIC FOR MELOIDOGYNE SPECIES, DNA VECTOR AND HOST CELL CONTAINING THE OLIGONUCLEOTIDE, USE AND KIT
(FR) OLIGONUCLEOTIDE D'ADN, SPECIFIQUE DE L'ESPECE MELOIDOGYNE, VECTEUR D'ADN ET CELLULE HOTE CONTENANT CET OLIGONUCLEOTIDE, UTILISATION ET TROUSSE
Abstract
(EN)
The invention relates to a DNA oligonucleotide which is specific for Meloidogyne species, which DNA oligonucleotide has the following sequence: (a) a DNA sequence chosen from the group consisting of SEQ ID No. 1 to SEQ ID No. 20, or (b) a DNA sequence which under conditions of stringency hybridises with a sequence according to (a) and which is specific for Meloidogyne species. Furthermore, the invention relates to a DNA vector containing such a DNA oligonucleotide, as well as to a host cell containing such vector. The invention also relates to a process for determining the presence of a Meloidogyne species, according to which DNA is isolated from a sample to be analysed, and is subsequently subjected to a PCR reaction, using a DNA oligonucleotide according to the invention. Finally, the invention relates to a kit for determining the presence of a Meloidogyne species.
(FR)
L'invention concerne un oligonucléotide d'ADN, spécifique de l'espèce Méloïdogyne, et possédant la séquence suivante: (a) une séquence d'ADN choisie dans le groupe constitué par SEQ ID No. 1 à SEQ ID No. 20, ou (b) une séquence d'ADN, s'hybridant, sous certaines conditions très précises, avec une séquence correspondant à a), et spécifique de l'espèce Méloïdogyne. En outre, l'invention concerne un vecteur d'ADN contenant un tel oligonucléotide, de même qu'une cellule hôte contenant ce vecteur. L'invention concerne encore un procédé de détermination de la présence d'une espèce Méloïdogyne, consistant à isoler l'ADN à partir d'un échantillon à analyser, et à le soumettre ensuite à une réaction d'amplification PCR, au moyen de l'oligonucléotide d'ADN de l'invention. Enfin, l'invention concerne une trousse de détermination de la présence d'une espèce Méloïdogyne.
Also published as
Latest bibliographic data on file with the International Bureau