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1. WO2000040735 - CONSTRUCT OF RECOMBINANT NUCLEIC ACIDS CIRCULARISED BY DNA COMPACTING AGENTS

Publication Number WO/2000/040735
Publication Date 13.07.2000
International Application No. PCT/FR2000/000004
International Filing Date 04.01.2000
Chapter 2 Demand Filed 05.07.2000
IPC
C12N 15/66 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
CPC
C12N 15/66
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Applicants
  • CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) [FR/FR]; 3, rue Michel Ange F-75794 Parix Cedex 16, FR (AllExceptUS)
  • AMOUYAL, Michèle [FR/FR]; FR (UsOnly)
Inventors
  • AMOUYAL, Michèle; FR
Agents
  • BREESE, Pierre ; Breese-Majerowicz 3, avenue de l'Opéra F-75001 Paris, FR
Priority Data
99/0014808.01.1999FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) CONSTRUCT OF RECOMBINANT NUCLEIC ACIDS CIRCULARISED BY DNA COMPACTING AGENTS
(FR) CONSTRUCTION D'ACIDES NUCLEIQUES RECOMBINANTS CIRCULARISES AU MOYEN D'AGENTS COMPACTANT L'ADN
Abstract
(EN)
The invention concerns a method for preparing circularised recombinant nucleic acids, consisting of a carrier and an insert capable of having a size greater than a few kb. The invention also concerns kits for preparing said nucleic acids and the recombinant nucleic acids obtainable by said method.
(FR)
L'invention concerne un procédé pour la préparation d'acides nucléiques recombinants circularisés, du type constitués d'un vecteur et d'un insert pouvant être d'une taille supérieure à quelque kilobases. L'invention concerne également les kits pour la préparation desdits acides nucléiques ainsi que les acides nucléiques recombinants susceptibles d'être obtenus par ce procédé.
Also published as
Latest bibliographic data on file with the International Bureau