Processing

Please wait...

Settings

Settings

1. WO2000040717 - METHOD FOR THE CELLULAR HIGH-THROUGHPUT-DETECTION OF NUCLEAR RECEPTOR LIGAND INTERACTIONS

Publication Number WO/2000/040717
Publication Date 13.07.2000
International Application No. PCT/EP1999/010461
International Filing Date 29.12.1999
Chapter 2 Demand Filed 26.07.2000
IPC
C07K 14/47 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C07K 14/72 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
72for hormones
C07K 14/82 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
82Translation products from oncogenes
C12N 9/16 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
C12N 15/62 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
G01N 33/566 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
566using specific carrier or receptor proteins as ligand binding reagent
CPC
C07K 14/4705
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4702Regulators; Modulating activity
4705stimulating, promoting or activating activity
C07K 14/721
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
72for hormones
721Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
C07K 14/82
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
82Translation products from oncogenes
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C12N 15/62
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
C12N 9/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
Applicants
  • SIPPEL, Albrecht, E. [DE/DE]; DE
  • ZIMMERMANN, André [DE/DE]; DE
Inventors
  • SIPPEL, Albrecht, E.; DE
  • ZIMMERMANN, André; DE
Agents
  • GÖTTE, Anna; Scharnitzer Strasse 42 82166 Gräfelfing, DE
Priority Data
198 60 834.930.12.1998DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) METHODE ZUR ZELLULÄREN HIGH-THROUGHPUT-DETEKTION VON NUKLEÄREN REZEPTOR-LIGANDEN-INTERAKTIONEN
(EN) METHOD FOR THE CELLULAR HIGH-THROUGHPUT-DETECTION OF NUCLEAR RECEPTOR LIGAND INTERACTIONS
(FR) METHODE DE DETECTION CELLULAIRE A HAUT RENDEMENT D'INTERACTIONS ENTRE DES RECEPTEURS NUCLEAIRES ET DES LIGANDS
Abstract
(DE)
Die Erfindung betrifft Fusionsproteine, umfassend mindestens drei Domänen, wobei eine erste Domäne eine Membranlokalisierung des Fusionsproteins in einem zellulären Kontext vermittelt und eine zweite Domäne eine Ligandenbindungsfunktion eines nukleären Rezeptors aufweist oder mutmasslich aufweist, eine dritte Domäne eine Aktivität aufweist, die in der Lage ist, einen sich an ein Ras-Protein anschliessenden Signalweg in einer Zelle zu aktivieren, dadurch gekennzeichnet, dass bei fehlender Bindung oder, alternativ, bei Bindung von Ligand an die zweite Domäne die dritte Domäne ihre Aktivität zur Aktivierung eines sich an ein Ras-Protein anschliessenden Signalwegs in einer Zelle trotz Membranlokalisierung nicht ausüben kann. Sie umfasst darüber hinaus u.a. Zellen, die derartige Fusionsproteine umfassen, Assayverfahren unter Einsatz derartiger Zellen, die u.a. dazu dienen, spezifische Interaktionen zwischen Ligandenbindungsabschnitten nukleärer Rezeptoren und Liganden zu detektieren, sowie Kits zur Verwendung in diesen Assays.
(EN)
The invention relates to fusion proteins comprising at least three domains, whereby the first domain mediates a membrane localisation of the fusion protein in a cellular context and the second domain is or might be provided with a ligand binding function of a nuclear receptor and a third domain can activate a signal path in a cell, whereby the signal path connects to a RAS protein. The invention is characterised in that a third domain cannot activate a RAS protein-connected signal path of a cell in spite of membrane localisation when the binding is missing or when the ligand is bonded to the second domain. The invention also relates to cells which comprise fusion proteins of that kind, to assay tests using said cells which can detect specific interactions between ligand binding sections of nuclear receptors and ligands. The invention further relates to kits for using said assays.
(FR)
L'invention concerne des protéines de fusion, comportant au moins trois domaines: le premier induit une localisation membranaire de la protéine de fusion dans un contexte cellulaire, le deuxième présente ou est supposé présenter une fonction de liaison de ligand d'un récepteur nucléaire, le troisième domaine présente une activité qui peut activer une voie de signalisation liée à une protéine Ras dans une cellule. L'invention est caractérisée en ce que, en cas de liaison manquante, ou en variante, en cas de liaison de ligand au deuxième domaine, le troisième domaine ne peut exercer son activité, à savoir activer une voie de signalisation liée à une protéine Ras, dans une cellule, en dépit d'une localisation membranaire. L'invention concerne en outre des cellules qui contiennent de telles protéines de fusion, des méthodes d'analyse au moyen de telles cellules, qui servent entre autres à détecter des interactions spécifiques entre des parties de liaison de ligand de récepteurs nucléaires et de ligands, ainsi que des kits utilisés dans ces analyses.
Latest bibliographic data on file with the International Bureau