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1. WO2000040715 - IMPROVED NUCLEIC ACID CLONING

Publication Number WO/2000/040715
Publication Date 13.07.2000
International Application No. PCT/US2000/000189
International Filing Date 05.01.2000
Chapter 2 Demand Filed 27.07.2000
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/66 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
CPC
C12N 15/1027
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
102Mutagenizing nucleic acids
1027by DNA shuffling, e.g. RSR, STEP, RPR
C12N 15/1093
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1093General methods of preparing gene libraries, not provided for in other subgroups
C12N 15/1096
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1096cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
C12N 15/66
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applicants
  • TRUSTEES OF BOSTON UNIVERSITY [US/US]; 108 Bay State Road Boston, MA 02215, US (AllExceptUS)
  • JARRELL, Kevin, A. [US/US]; US (UsOnly)
  • COLJEE, Vincent, W. [US/US]; US (UsOnly)
  • DONAHUE, William [US/US]; US (UsOnly)
  • MIKHEEVA, Svetlana [US/US]; US (UsOnly)
Inventors
  • JARRELL, Kevin, A.; US
  • COLJEE, Vincent, W.; US
  • DONAHUE, William; US
  • MIKHEEVA, Svetlana; US
Agents
  • JARRELL, Brenda, Herschbach; Choate, Hall & Stewart Exchange Place 53 State Street Boston, MA 02109, US
Priority Data
09/225,99005.01.1999US
60/114,90905.01.1999US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) IMPROVED NUCLEIC ACID CLONING
(FR) LIGATURE PERFECTIONNEE D'ACIDES NUCLEIQUES
Abstract
(EN)
The present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i. e., for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different products molecules are produced in a single ligation reaction. Preferred embodiments of the invention involved ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families.
(FR)
La présente invention se rapporte à un système perfectionné permettant de lier entre eux des acides nucléiques. Elle se rapporte notamment à des techniques de production de produits moléculaires d'ADN qui peuvent subir une ligature directe et facile avec des molécules réceptrices. Il n'est pas nécessaire que lesdits produits moléculaires subissent un clivage avec des enzymes de restriction aux fins de ladite ligature. Dans les réalisations préférées de l'invention, les produits moléculaires d'ADN sont obtenus au moyen de réactions de synthèse d'ADN itératives de manière que ces produits moléculaires sont des produits amplifiés. L'invention se rapporte en outre à la ligature directe de produits moléculaires (c'est-à-dire à la ligature sélective de certaines molécules appartenant à un ensemble de molécules), et également à des méthodes de redistribution d'exons, selon lesquelles de multiples produits moléculaires différents sont produits au cours d'une seule réaction de ligature. Les réalisations préférées de cette invention concernent la ligature de produits moléculaires codant des domaines de protéines fonctionnelles, et notamment de domaines qui se trouvent naturellement dans des familles de gènes conservés. Le système de manipulation d'ADN de cette invention peut être facilement intégré à d'autres systèmes de manipulation d'acides nucléiques, tels que les systèmes à médiation assurée par des ribozymes, et il peut en outre être facilement automatisé.
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