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1. WO2000040709 - PROCESS FOR ALTERING PLANT SYNTHESIS IN PLANTS USING MODIFIED TRNAS

Publication Number WO/2000/040709
Publication Date 13.07.2000
International Application No. PCT/IB1999/002125
International Filing Date 24.12.1999
Chapter 2 Demand Filed 19.07.2000
IPC
C12N 15/82 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C12N 15/8216
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
C12N 15/8251
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
C12N 15/8253
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
8253Methionine or cysteine
C12N 15/8254
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
8254Tryptophan or lysine
Applicants
  • FOLK, William, R. [US/US]; US
Inventors
  • FOLK, William, R.; US
Agents
  • CASTEL, Benoît; Young & Thompson 745 South 23rd Street Arlington, VA 22202, US
Priority Data
09/220,37524.12.1998US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PROCESS FOR ALTERING PLANT SYNTHESIS IN PLANTS USING MODIFIED TRNAS
(FR) PROCEDE PERMETTANT DE FAIRE CODER AUX PLANTES ET CELLULES DE PLANTES DE LA LYSINE OU DE LA METHIONINE OU DU TRYPTOPHANE OU DE LA THREONINE A DES CODONS D'ARNm DIFFERENTS DE CEUX SPECIFIANT NORMALEMENT CES ACIDES AMINES PARTICULIERS DURANT LA SYNTHESE PROTEIQUE
Abstract
(EN)
The present invention relates to a method for causing plant cells or plant tissue or plants to code lysine or methionine or tryptophan or threonine at mRNA codons other than those normally specifying those particular amino acids during protein synthesis, which comprises: i) preparing a DNA to express a tRNA which is charged with lysine and which binds to mRNA codons other than AAA or AAG during protein synthesis; or a DNA to express a tRNA which is charged with methionine and which binds to mRNA codons other than AUG; or a DNA to express a tRNA which is charged with tryptophan and which binds to mRNA codons other than UGG; or a DNA to express a tRNA which is charged with threonine and which binds to codons other than ACU, ACC, ACA, ACG; ii) introducing one or more of the said DNAs into plant protoplasts, plant cells or plants.
(FR)
La présente invention concerne un procédé permettant de faire coder aux cellules de plantes, ou aux tissus de plantes, de la lysine, ou de la méthionine, ou du tryptophane, ou de la thréonine, à des codons d'ARNm différents de ceux spécifiant normalement ces acides aminés particuliers durant la synthèse protéique. Ce procédé consiste: i) à préparer un ADN à exprimer un ARNt chargé en lysine qui se lie, lors de la synthèse protéique, à des codons d'ARNm autres que AAA ou AAG, ou à préparer un ADN qui exprime un ARNt chargé en méthionine qui se lie à des codons d'ARNm autres que AUG, ou à préparer un ADN qui exprime un ARNt chargé en tryptophane qui se lie à des codons d'ARNm autres que UGG, ou à préparer un ADN qui exprime un ARNt chargé en thréonine qui se lie à des codons d'ARNm autres que ACU, ACC, ACA, ACG, ii) et à introduire un ou plusieurs desdits ADN dans des protoplastes de plantes, dans des cellules de plantes, ou dans des plantes.
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