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1. WO2000039586 - TETRAMETHYLBENZIDINE FORMULATION FOR HORSERADISH PEROXIDASE ENZYME ASSAYS

Publication Number WO/2000/039586
Publication Date 06.07.2000
International Application No. PCT/US1999/031167
International Filing Date 29.12.1999
IPC
C12Q 1/28 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
26involving oxidoreductase
28involving peroxidase
CPC
C12Q 1/28
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
26involving oxidoreductase
28involving peroxidase
C12Q 2326/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2326Chromogens for determinations of oxidoreductase enzymes
10Benzidines
123,3',5,5'-Tetramethylbenzidine, i.e. TMB
Applicants
  • SEDUM LABORATORIES [US/US]; 51 Francis Avenue Mansfield, MA 02048, US (AllExceptUS)
  • WOISZWILLO, James, E. [US/US]; US (UsOnly)
Inventors
  • WOISZWILLO, James, E.; US
Agents
  • GREENE, Jamie, L. ; Jones & Askew, LLP 2400 Monarch Tower 3424 Peachtree Road, N.E. Atlanta, GA 30326, US
Priority Data
60/114,46030.12.1998US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) TETRAMETHYLBENZIDINE FORMULATION FOR HORSERADISH PEROXIDASE ENZYME ASSAYS
(FR) FORMULATION DE TETRAMETHYLBENZIDINE FAISANT MONTRE D'UNE EFFICACITE ACCRUE POUR DES DOSAGES DE L'ENZYME PEROXYDASE DU RAIFORT
Abstract
(EN)
A composition and method for detecting or measuring analyte in a sample. The composition is a tetraalkylbenzidine formulation in which a tetraalkylbenzidine chromogen is dissolved in a chromogen solvent that enhances the solubility and thereby increases the sensitivity of the chromogen when used in an assay, particularly an immunoassay. The chromogen solvent is acetronitrile. The tetraalkylbenzidine formulation is optionally diluted in an aqueous solution, such as a buffer.
(FR)
Cette invention a trait à une composition et à une méthode permettant de détecter ou de mesurer un analysat dans un échantillon. La composition est une formulation de tétraalkylbenzidine dans laquelle un chromogène de tétraalkylbenzidine est dissous dans un solvant de chromogène renforçant la solubilité et, partant, augmentant la sensibilité du chromogène lorsque celui-ci est utilisé dans un dosage, notamment dans un dosage immunologique. Le solvant de chromogène est un acétonitrile. La formulation de tétraalkylbenzidine est, éventuellement, diluée dans une solution aqueuse, un tampon notamment.
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