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1. WO2000039313 - PLASTID TRANSFORMATION OF $i(BRASSICA)

Publication Number WO/2000/039313
Publication Date 06.07.2000
International Application No. PCT/US1999/030183
International Filing Date 17.12.1999
Chapter 2 Demand Filed 21.07.2000
IPC
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C12N 15/8214
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8214Plastid transformation
Applicants
  • CALGENE LLC [US/US]; 1920 Fifth Street Davis, CA 95616, US
Inventors
  • CHAUDHURI, Sumita; US
  • OAKES, Janette, V.; US
Agents
  • SEARLE, G., D.; Monsanto Patent Dept. Central P.O. Box 5110 Chicago, IL 60680-5110, US
  • MCBRIDE, Thomas, P.; Monsanto Company 800 North Lindbergh Boulevard St. Louis, MO 63167, US
Priority Data
09/220,55723.12.1998US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PLASTID TRANSFORMATION OF $i(BRASSICA)
(FR) TRANSFORMATION DES PLASTES CHEZ LES $i(BRASSICA)
Abstract
(EN)
A method is provided for transforming $i(Brassica) plants to express DNA sequences of interest from the plant cell plastid. The method allows the transformation of $i(Brassica) plant tissue with heterologous DNA constructs. Such DNA constructs comprise, in the 5' to 3' direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The invention further provides for $i(Brassica) cells in which the plastids contain heterologous DNA constructs.
(FR)
L'invention concerne un procédé permettant de transformer des $i(Brassica) afin d'exprimer des séquences d'ADN d'intérêt provenant du plaste cellulaire desdites plantes. Ce procédé permet de transformer des tissus de $i(Brassica) au moyen de constructions d'ADN hétérologues. Ces constructions d'ADN comprennent, dans la direction 5'à 3' de la transcription, une zone de promoteur fonctionnelle dans un plaste, et une séquence d'ADN d'intérêt. L'invention concerne également des cellules de $i(Brassica) dans lesquelles les plastes contiennent des constructions d'ADN hétérologues.
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