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1. WO2000038743 - REMOVAL OF BIOLOGICAL CONTAMINANTS

Publication Number WO/2000/038743
Publication Date 06.07.2000
International Application No. PCT/AU1999/001171
International Filing Date 23.12.1999
Chapter 2 Demand Filed 31.05.2000
IPC
A61L 2/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
2Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
A61L 2/03 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
2Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
02using physical phenomena
03Electric current, e.g. electrolysis
C07K 1/26 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
24by electrochemical means
26Electrophoresis
CPC
A61L 2/0011
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
2Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
0005for pharmaceuticals, biologicals or living parts
0011using physical methods
A61L 2/0017
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
2Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
0005for pharmaceuticals, biologicals or living parts
0011using physical methods
0017Filtration
A61L 2/03
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
2Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
02using physical phenomena
03Electric current
B01D 57/02
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
57Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
02by electrophoresis
B01D 61/425
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
61Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis, ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
42Electrodialysis; Electro-osmosis ; Electro-ultrafiltration
425Electro-ultrafiltration
C07K 1/26
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14Extraction; Separation; Purification
24by electrochemical means
26Electrophoresis
Applicants
  • GRADIPORE LIMITED [AU/AU]; Riverside Corporate Park 35-105 Delhi Road North Ryde, NSW 2113, AU
Inventors
  • CONLAN, Brendon; AU
  • EDGELL, Tracy, Ann; AU
  • LAZAR, May; AU
  • NAIR, Chenicheri, Hariharan; AU
  • SEABROOK, Elizabeth, Jean; AU
  • TURTON, Thomas, Norman; AU
Agents
  • ALLENS PATENTS & TRADE MARK SERVICES; GPO Box 50 Sydney NSW 2001, AU
Priority Data
PP 790623.12.1998AU
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) REMOVAL OF BIOLOGICAL CONTAMINANTS
(FR) ELIMINATION DE CONTAMINANTS BIOLOGIQUES
Abstract
(EN)
A method of removing a biological contaminant from a mixture containing a biomolecule and the biological contaminant, the method comprising: (a) placing the biomolecule and contaminant mixture in a first solvent stream, the first solvent stream being separated from a second solvent stream by an electrophoretic membrane; (b) selecting a buffer for the first solvent stream having a required pH; (c) applying an electric potential between the two solvent streams causing movement of the biomolecule through the membrane into the second solvent stream while the biological contaminant is substantially retained in the first sample stream, or if entering the membrane, being substantially prevented from entering the second solvent stream; (d) optionally, periodically stopping and reversing the electric potential to cause movement of any biological contaminants having entered the membrane to move back into the first solvent stream, wherein substantially not causing any biomolecules that have entered the second solvent stream to re-enter first solvent stream; and (e) maintaining step (c), and optional step (d) if used, until the second solvent stream contains the desired purity of biomolecule.
(FR)
L'invention concerne une méthode d'élimination d'un contaminant biologique d'un mélange contenant une biomolécule et le contaminant biologique, la méthode consistant: (a) à placer le mélange de la biomolécule et du contaminant dans un premier flux de solvant, le premier flux de solvant étant séparé d'un second flux de solvant par une membrane électrophorétique; (b) à sélectionner un tampon pour le premier flux de solvant ayant un pH requis; (c) à appliquer un potentiel électrique entre les deux flux de solvant provoquant le mouvement de la biomolécule à travers la membrane jusque dans le second flux de solvant alors que le contaminant biologique est sensiblement retenu dans le premier flux d'échantillon, ou s'il pénètre la membrane, étant sensiblement empêché de pénétrer dans le second flux de solvant; (d) facultativement, à arrêter et à inverser périodiquement le potentiel électrique afin de provoquer le mouvement d'éventuels contaminants biologiques ayant pénétré dans la membrane afin de revenir dans le premier flux de solvant, de manière à ne pas faire rentrer dans le premier flux de solvant d'éventuelles molécules ayant pénétré dans le second flux de solvant; et (e) à maintenir l'étape (c), et une étape facultative (d) si elle est utilisée, jusqu'à ce que le second flux de solvant contienne la biomolécule à la pureté voulue.
Also published as
EP2006010053
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