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1. WO2000037674 - NUCLEIC ACID SEQUENCING METHOD

Publication Number WO/2000/037674
Publication Date 29.06.2000
International Application No. PCT/GB1999/004208
International Filing Date 20.12.1999
Chapter 2 Demand Filed 25.05.2000
IPC
C07H 19/04 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
19Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro derivatives thereof
02sharing nitrogen
04Heterocyclic radicals containing only nitrogen as ring hetero atom
C07H 21/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C07H 19/04
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
19Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides ; Anhydro-derivatives thereof
02sharing nitrogen
04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/6818
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6818involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
Applicants
  • THE VICTORIA UNIVERSITY OF MANCHESTER [GB]/[GB] (AllExceptUS)
  • DOUGLAS, Kenneth, Thomas [GB]/[GB] (UsOnly)
  • BICHENKOVA, Elena, Vladimirovna; [LV]/[GB] (UsOnly)
Inventors
  • DOUGLAS, Kenneth, Thomas
  • BICHENKOVA, Elena, Vladimirovna;
Agents
  • ATKINSON, Peter, Birch
Priority Data
9827908.619.12.1998GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) NUCLEIC ACID SEQUENCING METHOD
(FR) PROCEDE DE SEQUENÇAGE DE L'ACIDE NUCLEIQUE
Abstract
(EN)
A method of determining the sequence of a nucleic acid involves the use of an excimer or exciplex as a label. The method may comprise effecting a single copying reaction of the nucleic acid to be sequenced using chain extending nucleotides and an appropriate number of chain terminating nucleotides each labelled with the partners of a detectably different excimer or exciplex, the method further comprising separating the labelled fragments produced and detecting the labels to determine the sequence of the nucleic acid.
(FR)
L'invention concerne un procédé permettant de déterminer la séquence d'un acide nucléique, qui implique l'utilisation, comme marqueur, d'un excimère ou d'un exciplexe. Le procédé peut consister à effectuer une réaction de copiage simple de l'acide nucléique à séquencer au moyen de nucléotides d'allongement de chaîne et d'un nombre approprié de nucléotides de terminaison de chaîne, chacun marqué par des partenaires d'un excimère ou d'un exciplexe différent de manière détectable. Le procédé consiste également à séparer les fragments marqués produits et à détecter les marqueurs afin de déterminer la séquence de l'acide nucléique.
Also published as
US09868307
Latest bibliographic data on file with the International Bureau