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1. WO2000034435 - $i(CIS)-ELEMENT REPORTER CONSTRUCTS AND USES THEREOF

Publication Number WO/2000/034435
Publication Date 15.06.2000
International Application No. PCT/US1999/028451
International Filing Date 01.12.1999
IPC
C07K 14/47 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
CPC
C07K 14/4702
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4702Regulators; Modulating activity
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
Applicants
  • CLONTECH LABORATORIES, INC. [US]/[US]
Inventors
  • LI, Xianqiang
  • ZHAO, Xiaoning
  • JIANG, Xin
  • FANG, Yu
  • DUONG, Tommy
  • KAIN, Steve
Agents
  • ADLER, Benjamin, A.
Priority Data
09/206,88708.12.1998US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) $i(CIS)-ELEMENT REPORTER CONSTRUCTS AND USES THEREOF
(FR) CONSTRUCTIONS RAPPORTEURS A ELEMENTS CIS ET UTILISATIONS CORRESPONDANTES
Abstract
(EN)
The present invention provides $i(cis) element-reporter constructs for measuring transcription. A series of $i(cis) elements are linked to a reporter gene to generate $i(cis)-acting reporters. Examples of reporter genes are secreted alkaline phosphatase (SEAP), destabilized green fluorescent protein (d2EGFP) and luciferase. Also provided are various applications of such constructs.
(FR)
L'invention concerne des constructions rapporteurs d'éléments circuits intégrés permettant de mesurer la transcription. Une série d'éléments $i(cis) sont liés à un gène rapporteur afin de générer des rapporteurs à activité $i(cis). La phosphatase alcaline sécrétée (SEAP), la protéine verte fluorescente déstabilisée (d2EGFP) et la luciferase sont des exemples de gènes rapporteurs. L'invention concerne également différentes applications de ces constructions.
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