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1. WO2000032762 - MAGNETIC PARTICLES FOR PURIFYING NUCLEIC ACIDS

Publication Number WO/2000/032762
Publication Date 08.06.2000
International Application No. PCT/EP1999/008996
International Filing Date 23.11.1999
Chapter 2 Demand Filed 09.02.2000
IPC
B03C 1/01 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
1Magnetic separation
005Pretreatment specially adapted for magnetic separation
01by addition of magnetic adjuvants
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
H01F 1/00 2006.01
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
1Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
H01F 1/11 2006.01
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
1Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
01of inorganic materials
03characterised by their coercivity
032of hard-magnetic materials
10non-metallic substances, e.g. ferrites
11in the form of particles
CPC
B03C 1/01
BPERFORMING OPERATIONS; TRANSPORTING
03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
1Magnetic separation
005Pretreatment specially adapted for magnetic separation
01by addition of magnetic adjuvants
B82Y 25/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
25Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
C12N 15/1006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
C12N 15/1013
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
1013by using magnetic beads
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
H01F 1/11
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
1Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
01of inorganic materials
03characterised by their coercivity
032of hard-magnetic materials
10non-metallic substances, e.g. ferrites ; , e.g. [(Ba,Sr)O(Fe2O3)6] ferrites with hexagonal structure
11in the form of particles
Applicants
  • ROCHE DIAGNOSTICS GMBH [DE]/[DE] (AllExceptUS)
  • HARTTIG, Herbert [DE]/[DE] (UsOnly)
  • RIEDLING, Michael [DE]/[DE] (UsOnly)
  • MENNIG, Martin [DE]/[DE] (UsOnly)
  • SCHMIDT, Helmut [DE]/[DE] (UsOnly)
Inventors
  • HARTTIG, Herbert
  • RIEDLING, Michael
  • MENNIG, Martin
  • SCHMIDT, Helmut
Common Representative
  • ROCHE DIAGNOSTICS GMBH
Priority Data
198 54 973.330.11.1998DE
198 55 259.930.11.1998DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) MAGNETISCHE PARTIKEL ZUR REINIGUNG VON NUKLEINSÄUREN
(EN) MAGNETIC PARTICLES FOR PURIFYING NUCLEIC ACIDS
(FR) PARTICULES MAGNETIQUES POUR PURIFIER DES ACIDES NUCLEIQUES
Abstract
(DE)
Zubereitungen von Partikeln mit einer Glasoberfläche, wobei mehr als 75 Gew.-% dieser Partikel eine Korngröße von zwischen 0.5 $g(m)m und 15 $g(m)m haben sowie mit einer Glasoberfläche, welche einen Anteil von zwischen 2 und 6 mol-% Zinkoxid enthält, haben sich als besonders vorteilhaft in Nukleinsäurereinigungsverfahren erwiesen. Insbesondere wird eine erhöhte Nukleinsäureausbeute erreicht.
(EN)
The invention relates to preparations of particles having a glass surface. More than 75 wt.-% of said particles have a grain size of between 0.5 and 15 $g(m)m and have a glass surface which contains between 2 to 6 mole-% zinc oxide. The inventive particles are especially useful for purifying nucleic acids; particularly, an increased nucleic acid yield is achieved.
(FR)
L'invention concerne des préparations de particules ayant une surface en verre. Plus de 75 % en poids de ces particules ont une dimension granulaire comprise entre 0,5 et 15 $g(m)m, ainsi qu'une surface en verre contenant entre environ 2 et 6 % en mole d'oxyde de zinc. L'utilisation de ces préparations s'est avérée particulièrement avantageuse dans des procédés de purification d'acide nucléique. Ce procédé permet notamment de parvenir à de meilleurs rendements en acide nucléique.
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