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1. WO2000032748 - GENE NETWORKS FOR CONTROL OF GENE EXPRESSION

Publication Number WO/2000/032748
Publication Date 08.06.2000
International Application No. PCT/US1999/028592
International Filing Date 01.12.1999
Chapter 2 Demand Filed 09.06.2000
IPC
C12N 15/63 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
CPC
C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/635
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
635Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
Applicants
  • TRUSTEES OF BOSTON UNIVERSITY [US]/[US] (AllExceptUS)
  • GARDNER, Timothy, S. [US]/[US] (UsOnly)
  • COLLINS, James, J. [US]/[US] (UsOnly)
Inventors
  • GARDNER, Timothy, S.
  • COLLINS, James, J.
Agents
  • EISENSTEIN, Ronald, I.
Priority Data
60/110,61602.12.1998US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) GENE NETWORKS FOR CONTROL OF GENE EXPRESSION
(FR) RESEAUX DE GENES DESTINES A CONTROLER L'EXPRESSION GENETIQUE
Abstract
(EN)
The present invention relates to compositions for regulating gene expression in a cell. In particular, the invention provides composition constructs which switch expression of a gene of interest between stable 'on' or 'off' states in response to a transiently applied agent. The invention further provides adjustable-threshold switch constructs for expressing a gene of interest in response to the sustained application of an agent at a concentration above or below a desired threshold concentration. The invention also provides multi-state oscillator constructs in which expression of a gene of interest is periodically altered in the absence of administration of agents which are extraneous to the construct. Also provided are methods for using the invention's constructs.
(FR)
L'invention concerne des compositions destinées à réguler l'expression génétique dans une cellule. Elle concerne notamment des structures de compositions qui commutent l'expression d'un gène d'intérêt entre les états stables 'activé' et 'désactivé' en réponse à un agent appliqué de façon transitoire. L'invention concerne en outre un seuil réglable d'un agent à des concentrations supérieures ou inférieures à une concentration de seuil désirée. En outre, l'invention concerne des structures d'oscillateur à états multiples dans lesquelles l'expression d'un gène d'intérêt est périodiquement altérée en l'absence d'administration d'agents étrangers à la structure. L'invention concerne enfin des procédés d'utilisation des structures de l'invention.
Also published as
US09872338
US09872339
US09872868
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