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Machine translation
1. (WO2000026347) METHODS FOR THE PRODUCTION OF TCR GAMMA DELTA T CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2000/026347    International Application No.:    PCT/CA1999/001024
Publication Date: 11.05.2000 International Filing Date: 04.11.1999
Chapter 2 Demand Filed:    30.05.2000    
IPC:
A61K 35/12 (2006.01), A61K 35/14 (2006.01), A61K 35/24 (2006.01), A61K 35/26 (2006.01), A61K 35/36 (2006.01), A61K 35/407 (2006.01), A61P 31/00 (2006.01), A61P 35/00 (2006.01), A61P 35/02 (2006.01), A61P 37/02 (2006.01), A61P 43/00 (2006.01), C12N 5/0783 (2010.01), C12R 1/91 (2006.01)
Applicants: HEMOSOL INC. [CA/CA]; 115 Skyway Avenue, Etobicoke, Ontario M9W 4Z4 (CA) (For All Designated States Except US).
BELL, David, Nicholson [CA/CA]; (CA) (For US Only).
SKEA, Danna, Lynn [CA/CA]; (CA) (For US Only).
HEDGE, Phyllis, Robin [CA/CA]; (CA) (For US Only)
Inventors: BELL, David, Nicholson; (CA).
SKEA, Danna, Lynn; (CA).
HEDGE, Phyllis, Robin; (CA)
Agent: BERESKIN & PARR; 40 King Street West, 40th floor, Toronto, Ontario M5H 3Y2 (CA)
Priority Data:
60/107,006 04.11.1998 US
Title (EN) METHODS FOR THE PRODUCTION OF TCR GAMMA DELTA T CELLS
(FR) METHODES DE PRODUCTION DE LYMPHOCYTES T TCR$g(g)$g(d)
Abstract: front page image
(EN)The method for obtaining and expanding TcR$g(g)$g(d)?+¿ T cells in culture is described. The method involves: 1) culturing cells from a sample containing TcR$g(g)$g(d)?+¿ T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two cytokines and 2) culturing the cells obtained in step 1) in a second culture medium comprising at least two cytokines. Preferably, the method comprises 1) culturing the cells in a first culture medium comprising (a) a T cell mitogen, (b) interleukin-2 and (c) interleukin-4; and 2) culturing the cells obtained in step 1) in a second culture medium comprising (i) interleukin-2 and (ii) interleukin-4 to obtain TcR$g(g)$g(d)?+¿ T cells. The TcR$g(g)$g(d)?+¿ T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.
(FR)L'invention concerne une méthode permettant d'obtenir et de développer des lymphocytes T TcR$g(g)$g(d)?+¿ de culture. Ladite méthode consiste: 1) à cultiver les cellules à partir d'un échantillon renfermant des lymphocytes T TcR$g(g)$g(d)?+¿, ou leurs précurseurs, dans un premier milieu de culture contenant un mitogène des lymphocytes T et au moins deux cytokines et 2) à cultiver les cellules obtenues lors de l'étape 1) dans un second milieu de culture contenant au moins deux cytokines. De préférence, la méthode consiste 1) à cultiver les cellules dans un premier milieu de culture renfermant (a) un mitogène des lymphocytes T, (b) une interleukine-2 et (c) une interleukine-4; et 2) à cultiver les cellules obtenues à l'étape 1) dans un second milieu de culture renfermant (i) une interleukine-2 et (ii) une interleukine-4, de manière à obtenir des lymphocytes T TcR$g(g)$g(d)?+¿. Les lymphocytes T TcR$g(g)$g(d)?+¿ obtenus par cette méthode peuvent être utilisés dans diverses applications expérimentales, thérapeutiques et commerciales.
Designated States: AE, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CR, CU, CZ, DE, DK, DM, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)