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1. WO2000024910 - PROCESS OF PURIFICATION OF LOW DENSITY LIPOPROTEIN ASSOCIATED PHOSPHOLIPASE A2 USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY

Publication Number WO/2000/024910
Publication Date 04.05.2000
International Application No. PCT/GB1999/003551
International Filing Date 27.10.1999
Chapter 2 Demand Filed 16.05.2000
IPC
C12N 9/18 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
18Carboxylic ester hydrolases
CPC
B01D 15/3828
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
38involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
3804Affinity chromatography
3828Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
C12N 2799/026
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2799Uses of viruses
02as vector
021for the expression of a heterologous nucleic acid
026where the vector is derived from a baculovirus
C12N 9/18
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
18Carboxylic ester hydrolases ; (3.1.1)
Applicants
  • SMITHKLINE BEECHAM PLC [GB]/[GB] (AllExceptUS)
  • BOYD, Helen [GB]/[GB] (UsOnly)
  • TEW, David, Graham [GB]/[US] (UsOnly)
Inventors
  • BOYD, Helen
  • TEW, David, Graham
Agents
  • CONNELL, Anthony, Christopher
  • GIDDINGS, Peter John
Priority Data
9823647.428.10.1998GB
9823648.228.10.1998GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PROCESS OF PURIFICATION OF LOW DENSITY LIPOPROTEIN ASSOCIATED PHOSPHOLIPASE A2 USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
(FR) PROCEDE DE PURIFICATION DE PHOSPHOLIPASE A2 ASSOCIEE A UNE LIPOPROTEINE DE FAIBLE DENSITE (LDL-PLA2) AU MOYEN D'UNE CHROMATOGRAPHIE PAR AFFINITE AVEC UN METAL IMMOBILISE
Abstract
(EN)
A novel process for the purification of recombinant LDL-PLA2. Uses of the purified LDL-PLA2 so obtained are also disclosed.
(FR)
L'invention concerne un nouveau procédé de purification de LDL-PLA2 recombinant, ainsi que des utilisations du LDL-PLA2 purifié ainsi obtenu.
Also published as
US09830395
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