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1. WO2000023580 - A METHOD OF AFFINITY SEPARATION AND LIGANDS FOR USE THEREIN

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Publication Number WO/2000/023580
Publication Date 27.04.2000
International Application No. PCT/GB1999/003484
International Filing Date 21.10.1999
Chapter 2 Demand Filed 22.05.2000
IPC
C07K 1/22 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
16by chromatography
22Affinity chromatography or related techniques based upon selective absorption processes
C07K 14/31 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
305from Micrococcaceae (F)
31from Staphylococcus (G)
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
C07K 1/047
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
04on carriers
047Simultaneous synthesis of different peptide species; Peptide libraries
C07K 1/22
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14Extraction; Separation; Purification
16by chromatography
22Affinity chromatography or related techniques based upon selective absorption processes
C07K 14/31
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
305from Micrococcaceae (F)
31from Staphylococcus (G)
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C12N 15/1037
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
C40B 40/02
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40Libraries per se, e.g. arrays, mixtures
02Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
Applicants
  • AFFIBODY TECHNOLOGY SWEDEN AB [SE]/[SE] (AllExceptUS)
  • GARDNER, Rebecca [GB]/[GB] (GB)
  • UHLÉN, Mathias [SE]/[SE] (UsOnly)
  • HOBER, Sophia [SE]/[SE] (UsOnly)
Inventors
  • UHLÉN, Mathias
  • HOBER, Sophia
Agents
  • GARDNER, Rebecca
Priority Data
9823071.721.10.1998GB
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) A METHOD OF AFFINITY SEPARATION AND LIGANDS FOR USE THEREIN
(FR) PROCEDE DE SEPARATION PAR AFFINITE ET LIGANDS UTILISES DANS CE PROCEDE
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Abstract
(EN) The present invention relates to methods of affinity separation wherein the affinity ligand is an immobilised proteinaceous ligand wherein one or more of its asparagine (Asn) residues has been modified. Methods of making a stabilised combinatorial protein comprising the steps of: a) modification of Asn residues within a protein molecule to increase stability of the protein in alkaline conditions; and b) randomisation of the protein molecule to modify its binding characteristics and combinatorial proteins wherein in a step separate from the randomisation step, the stability of the protein in alkaline conditions has been increased by modifying one or more of its Asn residues are also described.
(FR) Cette invention se rapporte à des procédés de séparation par affinité, dans lesquels le ligand d'affinité est constitué par un ligand protéiné immobilisé, dont un ou plusieurs de ses restes'asparagine (Asn) ont été modifiés. Les procédés de formation d'une protéine combinatoire stabilisée consistent: (a) à modifier des résidus Asn à l'intérieur d'une molécule de protéine pour accroître la stabilité de la protéine dans des conditions alcalines; (b) et à randomiser la molécule de cette protéine pour modifier ses caractéristiques de liaison. Cette invention présente également des protéines combinatoires pour lesquelles, dans une étape séparée de l'étape de randomisation, la stabilité de la protéine dans des conditions alcalines a été augmentée par modification d'un ou de plusieurs de ses résidus Asn.
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