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1. WO2000016062 - METHODS FOR DETERMINING THE ACTIVITY OF COMPLEX MIXTURES

Publication Number WO/2000/016062
Publication Date 23.03.2000
International Application No. PCT/US1999/021849
International Filing Date 17.09.1999
Chapter 2 Demand Filed 17.04.2000
IPC
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 2500/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2500Screening for compounds of potential therapeutic value
G01N 33/5008
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
5008for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
G01N 33/5023
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
5008for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
502for testing non-proliferative effects
5023on expression patterns
G01N 33/505
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
5008for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
5044involving specific cell types
5047Cells of the immune system
505involving T-cells
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/6842
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
Applicants
  • BIONEXUS [US]/[US]
Inventors
  • BABISH, John, G.
  • PACIORETTY, Linda, M.
  • LEE, M., Lisa
Agents
  • SINDER, Stuart, J.
Priority Data
09/156,74117.09.1998US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR DETERMINING THE ACTIVITY OF COMPLEX MIXTURES
(FR) TECHNIQUES PERMETTANT DE DETERMINER L'ACTIVITE DE MELANGES COMPLEXES
Abstract
(EN)
According to the present invention, the biological or pharmacological activity of a test material like a plant or herbal material, an extract of a plant or herbal material, a natural or synthetic compound or some combination thereof, can be quantified by observing the pattern of structural changes induced in a eukaryotic cell's proteins. These structural changes may be evidenced by protein phosphorylation, by protein-protein interactions and the like. The amount and nature of protein phosphorylation is qualitatively and quantitatively related to the in vitro concentration of biologically/pharmacologically active components to which the mammalian cells are exposed. Additionally, formation or loss of protein-protein complexes may be determined in whole cell homogenates through the use of nondenaturing electrophoresis and staining for proteins or protein phosphorylation. The present invention allows natural products to be formulated into nutritional supplements and pharmacological preparations of consistent biological/pharmacological activity without the need to identify any of the chemical constituents responsible for the biological or pharmacological response.
(FR)
Selon la présente invention, il est possible de quantifier l'activité biologique ou pharmaceutique d'une substance à analyser, telle qu'une substance végétale ou à base d'herbes médicinales, un extrait de plante ou d'herbe médicinale, un composé naturel ou de synthèse ou une quelconque combinaison de ces produits par observation du schéma des changements structurels induits dans les protéines d'une cellule eucaryote. Ces changements structurels peuvent être mis en évidence par la phosphorylation des protéines, par les interactions protéine-protéine, etc. L'importance et la nature de la phosphorylation des protéines est associée qualitativement et quantitativement à la concentration $i(in vitro) en composants biologiquement/pharmaceutiquement actifs auxquels les cellules de mammifère sont exposées. En outre, la formation ou la disparition de complexes protéine-protéine peut être déterminée dans des homogénats de cellules entières par électrophorèse non dénaturante et par coloration des protéines ou par phosphorylation des protéines. La présente invention permet de formuler des produits naturels en des compléments nutritionnels et des préparations pharmacologiques présentant une activité biologique/pharmacologique stable sans qu'il soit besoin d'identifier l'un quelconque des composants chimiques responsables de la réaction biologique ou pharmacologique.
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