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1. WO2000015842 - METHOD OF ISOLATION PRIMER EXTENSION PRODUCTS WITH MODULAR OLIGONUCLEOTIDES

Publication Number WO/2000/015842
Publication Date 23.03.2000
International Application No. PCT/GB1999/003056
International Filing Date 15.09.1999
Chapter 2 Demand Filed 14.04.2000
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6832
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6832Enhancement of hybridisation reaction
C12Q 1/6834
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
Applicants
  • DYNAL ASA [NO]/[NO] (AllExceptUS)
  • JONES, Elizabeth, Louise [GB]/[GB] (GB)
  • LUNDEBERG, Joakim [SE]/[SE] (UsOnly)
  • UHLÉN, Mathias [SE]/[SE] (UsOnly)
Inventors
  • LUNDEBERG, Joakim
  • UHLÉN, Mathias
Agents
  • JONES, Elizabeth, Louise
Priority Data
09/153,24215.09.1998US
9820185.816.09.1998GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF ISOLATION PRIMER EXTENSION PRODUCTS WITH MODULAR OLIGONUCLEOTIDES
(FR) PROCEDE D'ISOLATION DE PRODUITS D'EXTENSION D'AMORCES AU MOYEN D'OLIGONUCLEOTIDES MODULAIRES
Abstract
(EN)
The invention provides a method of isolating primer extension products, particularly sequencing reaction products, in which the products contain sequences corresponding or complementary to i) a primer binding region, ii) an insert and iii) vector-derived sequence(s), and a modular oligonucleotide of at least two parts which is complementary to the vector-derived sequence(s) and binds to adjacent stretches of the primer extension products is used for isolation, the modular oligonucleotides themselves and their use in methods of the invention.
(FR)
L'invention concerne un procédé d'isolation de produits d'extension d'amorces, notamment de produits de réaction de séquençage, lesquels produits contiennent des séquences correspondant à ou complémentaire de i) une région de fixation d'amorce, ii) un segment d'insertion et iii) une (des) séquence(s) dérivées de vecteurs ; elle concerne également l'utilisation, pour cette isolation, d'un oligonucléotide modulaire, lequel comprend au moins deux parties, est complémentaire de la (des) séquence(s) dérivées de vecteurs et se fixe sur des fragments adjacents des produits d'extension d'amorces. L'invention concerne également ces oligonucléotides ainsi que l'utilisation de ceux-ci dans des procédés de l'invention.
Also published as
US09787275
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