Processing

Please wait...

Settings

Settings

1. WO1999049082 - MODIFIED NUCLEOTIDES AND METHODS USEFUL FOR NUCLEIC ACID SEQUENCING

Publication Number WO/1999/049082
Publication Date 30.09.1999
International Application No. PCT/US1999/006329
International Filing Date 23.03.1999
Chapter 2 Demand Filed 22.10.1999
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C12Q 1/68 (2006.01)
CPC
C12Q 1/6869
Applicants
  • INVITROGEN CORPORATION [US/US]; 1600 Faraday Avenue Carlsbad, CA 92008, US
Inventors
  • SHORT, Jay, M.; US
Agents
  • BURKE, John, E. ; Pillsbury Madison & Sutro LLP 1100 New York Avenue, N.W. Washington, DC 20005, US
Priority Data
60/078,96923.03.1998US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MODIFIED NUCLEOTIDES AND METHODS USEFUL FOR NUCLEIC ACID SEQUENCING
(FR) NUCLEOTIDES MODIFIES ET METHODES UTILES POUR LE SEQUENÇAGE D'ACIDES NUCLEIQUES
Abstract
(EN)
The present invention relates to a method for sequencing nucleic acids. In particular, such method includes incorporating certain modified nucleotides referred to as Simtides into nucleic acid strands. These Simtides are complexes comprising a nucleotide base (or analog thereof), a linker, and a label capable of generating a detectable signal for sequencing. A Simtide may be incorporated into a sequencing fragment as a primer component, as a chain elongator, or as a chain terminator.
(FR)
La présente invention concerne une méthode de séquençage d'acides nucléiques. En particulier, cette méthode consiste à incorporer certains nucléotides modifiés appelés Simtides dans des brins d'acides nucléiques. Ces Simtides sont des complexes comprenant une base nucléotidique (ou analogue de celle-ci), un lieur, ainsi qu'une marque capable de produire un signal détectable pour le séquençage. Un Simtide peut être incorporé dans un fragment de séquençage en tant que constituant d'amorce, et un prolongateur de chaîne, ou un terminateur de chaîne.
Also published as
Latest bibliographic data on file with the International Bureau