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Machine translation
1. (WO1999041410) METHODS FOR DNA AMPLIFICATION AND SEQUENCING
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1999/041410    International Application No.:    PCT/IL1999/000080
Publication Date: 19.08.1999 International Filing Date: 08.02.1999
Chapter 2 Demand Filed:    19.08.1999    
IPC:
C12Q 1/68 (2006.01)
Applicants: YEDA RESEARCH AND DEVELOPMENT CO. LTD. [IL/IL]; Weizmann Institute of Science P.O. Box 95 76100 Rehovot (IL) (For All Designated States Except US).
LAPIDOT, Aviva [IL/IL]; (IL) (For US Only).
IAKOBASHVILI, Robert [IL/IL]; (IL) (For US Only).
MALIN, Gennady [IL/IL]; (IL) (For US Only)
Inventors: LAPIDOT, Aviva; (IL).
IAKOBASHVILI, Robert; (IL).
MALIN, Gennady; (IL)
Agent: BEN-AMI, Paulina; Yeda Research and Development Co. Ltd. Weizmann Institute of Science P.O. Box 95 76100 Rehovot (IL)
Priority Data:
123256 10.02.1998 IL
Title (EN) METHODS FOR DNA AMPLIFICATION AND SEQUENCING
(FR) PROCEDES D'AMPLIFICATION ET DE SEQUENCAGE D'ADN
Abstract: front page image
(EN)The osmoprotectants proline, 2- methyl- 4- carboxy- 3,4,5,6- tetrahydropyrimidine ('THP(B)'), and 2-methyl-4-carboxy-5-hydroxy-3,4,5,6-tetrahydropyrimidine ('THP(A)') are capable of increasing the thermal stability of DNA polymerases at elevated temperatures. THP(B) is further effective in lowering the melting temperature of double-stranded DNA. Proline, THP(A) and THP(B) are thus useful in procedures involving melting of double-stranded DNA and/or polymerase-mediated DNA synthesis, such as in primer extension, in PCR (polymerase chain reaction) amplification and in DNA sequencing.
(FR)Les osmoprotecteurs proline, 2- méthyl- 4- carboxy- 3,4,5,6- tétrahydropyrimidine ('THP(B)'), et 2-méthyl-4-carboxy-5-hydroxy-3,4,5,6-tétrahydropyrimidine ('THP(A)') sont capables d'augmenter la stabilité thermique d'ADN polymérases à des températures élevées. La THP(B) est en outre efficace pour abaisser la température de fusion d'ADN double brin. La proline, la THP(A) et la THP(B) sont donc utiles dans des procédures comportant la fusion de l'ADN double brin et/ou la synthèse d'ADN induite par polymérase, telles que l'extension d'amorces, l'amplification par PCR et le séquençage de l'ADN.
Designated States: CA, IL, JP, US.
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE).
Publication Language: English (EN)
Filing Language: English (EN)